Science of Brewing Archives - Brookston Beer Bulletin

Historic Beer Birthday: Hans Adolf Krebs

August 25, 2017 By Jay Brooks Leave a Comment

Today is the birthday of Hans Adolf Krebs (August 25, 1900-November 22, 1981). He was a German-born British physician and biochemist. He was the pioneer scientist in study of cellular respiration, a biochemical pathway in cells for production of energy. He is best known for his discoveries of two important chemical reactions in the body, namely the urea cycle and the citric acid cycle. The latter, the key sequence of metabolic reactions that produces energy in cells, often eponymously known as the “Krebs cycle,” earned him a Nobel Prize in Physiology or Medicine in 1953. And it’s the Krebs cycle that is his relation to brewing, as it’s also known as the respiratory phase, the second aerobic state of the fermentation process immediately following the lag period.

Here’s a description of the Krebs cycle from Life Fermented:

The Krebs cycle, also known as the tricarboxylic acid (TCA) cycle or the citric acid cycle, is a circular and repeating set of reactions which requires oxygen. In beer making, this would occur in the first stage of fermentation when the yeast is pitched into a well aerated wort, and carries on until all oxygen is used up.
Pyruvate (are you tired of this word yet?) is first converted to acetyl-CoA (pronounced “Co-A”) in the following reaction:

pyruvate + 2 NAD+ + CoA-SH → acetyl-CoA + CO2 + NADH, with the help of the pyruvate dehydrogenase (PDH) complex. Note that this is the first time CO2 is produced, and yet more NADH is generated.

This acetyl-CoA then enters into a cycle of reactions which nets two molecules of CO2, one GTP (guanosine triphosphate, another unit of energy equivalent to ATP), three NADH, and one FADH2 (flavin adenine dinucleotide, which functions similarly to NADH). After the cycle completes, another acetyl-CoA molecule enters and the cycle repeats itself.

But wait, this just made more NADH, and we need to regenerate NAD+ so glycolysis can continue. Both the NADH and FADH2 now donate their electrons to a process called the electron transport chain/ oxidative phosphorylation. The result is a return of NAD to the NAD+ state, and a large amount of ATP cellular energy.

Because the Krebs cycle is so efficient at producing ATP energy units, this is the yeast’s preferred pathway. But, you’ll notice a rather conspicuous absence: ethanol. This is only formed in the absence of oxygen.

Here’s a biography of Krebs, from the Nobel Prize website:

Sir Hans Adolf Krebs was born at Hildesheim, Germany, on August 25th, 1900. He is the son of Georg Krebs, M.D., an ear, nose, and throat surgeon of that city, and his wife Alma, née Davidson.

Krebs was educated at the Gymnasium Andreanum at Hildesheim and between the years 1918 and 1923 he studied medicine at the Universities of Göttingen, Freiburg-im-Breisgau, and Berlin. After one year at the Third Medical Clinic of the University of Berlin he took, in 1925, his M.D. degree at the University of Hamburg and then spent one year studying chemistry at Berlin. In 1926 he was appointed Assistant to Professor Otto Warburg at the Kaiser Wilhelm Institute for Biology at Berlin-Dahlem, where he remained until 1930.

In I930, he returned to hospital work, first at the Municipal Hospital at Altona under Professor L. Lichtwitz and later at the Medical Clinic of the University of Freiburg-im-Breisgau under Professor S. J. Thannhauser.

In June 1933, the National Socialist Government terminated his appointment and he went, at the invitation of Sir Frederick Gowland Hopkins, to the School of Biochemistry, Cambridge, where he held a Rockefeller Studentship until 1934, when he was appointed Demonstrator of Biochemistry in the University of Cambridge.

In 1935, he was appointed Lecturer in Pharmacology at the University of Sheffield, and in 1938 Lecturer-in-Charge of the Department of Biochemistry then newly founded there.

In 1945 this appointment was raised to that of Professor, and of Director of a Medical Research Council’s research unit established in his Department. In 1954 he was appointed Whitley Professor of Biochemistry in the University of Oxford and the Medical Research Council’s Unit for Research in Cell Metabolism was transferred to Oxford.

Professor Krebs’ researches have been mainly concerned with various aspects of intermediary metabolism. Among the subjects he has studied are the synthesis of urea in the mammalian liver, the synthesis of uric acid and purine bases in birds, the intermediary stages of the oxidation of foodstuffs, the mechanism of the active transport of electrolytes and the relations between cell respiration and the generation of adenosine polyphosphates.

Among his many publications is the remarkable survey of energy transformations in living matter, published in 1957, in collaboration with H. L. Kornberg, which discusses the complex chemical processes which provide living organisms with high-energy phosphate by way of what is known as the Krebs or citric acid cycle.

Krebs was elected a Fellow of the Royal Society of London in 1947. In 1954 the Royal Medal of the Royal Society, and in 1958 the Gold Medal of the Netherlands Society for Physics, Medical Science and Surgery were conferred upon him. He was knighted in 1958. He holds honorary degrees of the Universities of Chicago, Freiburg-im-Breisgau, Paris, Glasgow, London, Sheffield, Leicester, Berlin (Humboldt University), and Jerusalem.

He married Margaret Cicely Fieldhouse, of Wickersley, Yorkshire, in 1938. They have two sons, Paul and John, and one daughter, Helen.

And in the Microbe Wiki, on a page entitled “Saccharomyces cerevisiae use and function in alcohol production,” under a section called “Fermentation of alchohol,” the Krebs cycle is placed in its portion in the fermentation process:

Saccharomyces cerevisiae is able to perform both aerobic and anaerobic respiration. The process begins with the yeast breaking down the different forms of sugar in the wort. The types of sugars typically found in wort are the monosaccharides glucose and fructose. These sugars contain a single hexose, which is composed of 6 carbon atoms in the molecular formula C6H12O6. Disaccharides are formed when two monosaccharides join together. Typical disaccharides in the wort are galactose, sucrose, and maltose. The third type of fermentable sugar in the wort is a trisaccharide. This trisaccharide is formed when three monosccharides join together. Maltotriose is the trisaccharide commonly found in the wort and is composed of three glucose molecules. The wort does contain other sugars such as dextrins but it is not fermentable by yeast10. These dextrins contain four monosaccarides joined together. In order for the yeast to use the disaccharides and trisaccharides they first must be broken down to monosaccharides. The yeast does this by using different enzymes both inside and outside the cell. The enzyme invertase is used to break down sucrose into glucose and fructose. The invertase catalyzes the hydrolysis of the sucrose by breaking the O-C (fructose bond). The other enzyme used is maltase, which breaks down maltose and maltotriose into glucose inside the cell. The enzyme does this by catalyzing the hydrolysis of the sugars by breaking the glycosidic bond holding the glucose molecules together.

Once the sugars are broken down into monosaccharides the yeast can use them. The primary step is called glycolysis. In this process the glucose is converted to pyruvate using different enzymes in a series of chemical modifications. The electrons from glucose end up being transferred to energy carrying molecules like NAD+ to form NADH. ATP is also formed when phosphates are transferred from high-energy intermediates of glycolysis to ADP. In the presence of oxygen aerobic respiration can occur. This occurs in the mitochondria of the yeast. The energy of the pyruvate is extracted when it goes through metabolic processes like the Krebs cycle. The products of this type of metabolism are ATP, H2O, and CO2. However if there is no oxygen present and an abundance of sugars, as in the wort, the yeast undergo alcoholic fermentation. This type of metabolism yields much smaller amounts of energy when compared to aerobic respiration. However, because of the large supply of sugars from the different grains the wort is a very good environment for fermentative growth. The alcoholic fermentation begins with the two pyruvate acquired from glycolysis. These two pyruvate are decarboxylated by pyruvate decarboxylase to form two acetaldehydes and CO2. The CO2 is the gas that is observed during fermentation as bubbles that float to the top of the wort creating the kräusen or beer head, the foam that is very characteristic of a freshly poured beer. Pyruvate decarboxylase is a homotetramer meaning it contains four identical subunits. This also means that is has four active sites. The active sites are where the pyruvate reacts with the cofactors thiamine pyrophosphate (TPP) and magnesium to remove the carbon dioxide9. The final step to form alcohol is the addition of a hydrogen ion to the aldehyde to form ethanol. This hydrogen ion is from the NADH made during glycolysis and converts back to NAD+. The ethanol is originally believed to serve as an antibiotic against other microbes. This form of defense ensures that bacteria do not grow in the wort, thus ruining the beer with off flavors. However recently with the boom of craft beer different bacteria have been purposefully added to create what is known as sour beer. The sour taste comes from the waste products of the bacteria.

To learn more about the Krebs cycle check out this video from the University of Oklahoma’s Chemistry of Beer – Unit 7 – Chemical Concepts: Krebs Cycle:

Historic Beer Birthday: Johan Kjeldahl

August 16, 2017 By Jay Brooks Leave a Comment

Today is the birthday of Johan Gustav Christoffer Thorsager Kjeldahl (August 16, 1849-July 18, 1900) He was a Danish chemist who developed a method for determining the amount of nitrogen in certain organic compounds using a laboratory technique which was named the Kjeldahl method after him.

Kjeldahl worked in Copenhagen at the Carlsberg Laboratory, associated with Carlsberg Brewery, where he was head of the Chemistry department from 1876 to 1900.

He was given the job to determine the amount of protein in the grain used in the malt industry. Less protein meant more beer. Kjeldahl found the answer was in developing a technique to determine nitrogen with accuracy but existing methods in analytical chemistry related to proteins and biochemistry at the time were far from accurate.

A painting by Otto Haslund of Johan Kjeldahl.

His discovery became known as the Kjeldahl Method

The method consists of heating a substance with sulphuric acid, which decomposes the organic substance by oxidation to liberate the reduced nitrogen as ammonium sulphate. In this step potassium sulphate is added to increase the boiling point of the medium (from 337 °C to 373 °C) . Chemical decomposition of the sample is complete when the initially very dark-coloured medium has become clear and colourless.

The solution is then distilled with a small quantity of sodium hydroxide, which converts the ammonium salt to ammonia. The amount of ammonia present, and thus the amount of nitrogen present in the sample, is determined by back titration. The end of the condenser is dipped into a solution of boric acid. The ammonia reacts with the acid and the remainder of the acid is then titrated with a sodium carbonate solution by way of a methyl orange pH indicator.

In practice, this analysis is largely automated; specific catalysts accelerate the decomposition. Originally, the catalyst of choice was mercuric oxide. However, while it was very effective, health concerns resulted in it being replaced by cupric sulfate. Cupric sulfate was not as efficient as mercuric oxide, and yielded lower protein results. It was soon supplemented with titanium dioxide, which is currently the approved catalyst in all of the methods of analysis for protein in the Official Methods and Recommended Practices of AOAC International.

And Velp Scientifica also has an explanation of his method, which is still in use today.

Kjeldahl (center) in his laboratory.

Historic Beer Birthday: Max Henius

June 16, 2017 By Jay Brooks 4 Comments

Today is the birthday of Max Henius (June 16, 1859–November 15, 1935). He “was a Danish-American biochemist who specialized in the fermentation processes. Max Henius co-founded the American Academy of Brewing in Chicago.”

Max Henius, at left, at Rebild, Denmark.

Here’s his biography, from Wikipedia:

Max Henius was born in Aalborg, Denmark. His parents were Isidor Henius (1820–1901) and Emilie (née Wasserzug) Henius (1839–1913), both Polish Jewish immigrants. His father, who was born in Thorn, West Prussia, now Torun, Poland, emigrated to Denmark in 1837 and continued his work for spirits distillers to improve and standardise production and later – 15 January 1846 – co-founded one distillery, Aalborg priviligerede Sirup- og Sprtitfabrik, that was later, together with several other distilleries, consolidated into De Danske Spritfabrikker in 1881, a Danish distillery which is now – since 2012 – part of the Norwegian Arcus Group, which closed the distillery in Aalborg in 2015, moving production to Norway instead. Isidor Henius also owned a small castle in Aalborg, now called Sohngaardsholm Slot. Since 2005, it has been the site of a gourmet restaurant.

Max Henius was educated at the Aalborg Latin School and went on to study at the Polytechnic Institute in Hanover, Germany He attended the University of Marburg, earning his Ph.D. degree in chemistry during 1881. His father sold the distillery that same year. Max Henius subsequently emigrated from Aalborg to the United States in 1881 at the age of 22, settling in Chicago. His younger brother, Erik S. Henius, (1863- 1926) remained in Denmark where he was Chairman of the Danish Export Association.

Initially he was employed by the Northern Pacific Railway on an assignment to test the waters between Fargo, North Dakota, and Bozeman, Montana. In 1886, he opened a drug store. Subsequently he formed Wahl & Henius, an institute for chemical and mechanical analysis, with his former schoolmate, Robert Wahl (1858-1937). Founded in 1891, the Chicago-based American Brewing Academy (later known as the Wahl-Henius Institute of Fermentology) was one of the premier brewing schools of the pre-prohibition era. This institute was later expanded with a brew master school that operated until 1921.

At the beginning of the twentieth century, Max Henius became interested in Danish-American organizations in Chicago. Funds were being raised by Danish Americans to purchase 200 acres (0.81 km2) of heather-covered hills, located in part of Rold Forest (Danish: Rold Skov), Denmark’s largest forest. In 1912 Max Henius presented the deed to H.M. King Christian X as a permanent memorial from Danish Americans. Rebild National Park (Danish: Rebild Bakker) is today a Danish national park situated near the town of Skørping in Rebild municipality, Region Nordjylland in northern Jutland, Denmark. Every July 4 since 1912, except during the two world wars, large crowds have gathered in the heather-covered hills of Rebild to celebrate American Independence Day. On the slope north of Rebild, where the residence of Max Henius was once located, a bust was placed in his memory.

And here’s Randy Mosher’s entry from the Oxford Companion to Beer of the Wahl-Henius Institute of Fermentology:

Wahl-Henius Institute of Fermentology
is a brewing research laboratory and school in Chicago that operated between 1886 and 1921.

Founded in 1886 by Dr Robert Wahl and Dr Max Henius as the Wahl & Henius, the name was changed to the Scientific Station for Brewing of Chicago and then to the Institute of Fermentology before becoming the Wahl-Henius Institute. Its educational division, the American Brewing Academy, was created in 1891.

The school and laboratory operated successfully until Prohibition, when the near dissolution of the brewing trade forced its closure and sale to the American Institute of Baking, which retains the nucleus of the Wahl-Henius library.

Wahl-Henius would perhaps be mostly forgotten today if it were not for its role as publisher of two important beer texts. The Wahl-Henius Handy Book of Brewing, Malting and the Auxillary Trades, coauthored by Wahl and Henius, is a comprehensive and wide-ranging view into American brewing in 1901. It also contains basic chemical analyses of many contemporary American and European beers, providing an unusually valuable window into the brewing past. J. P. Arnold’s 1911 Origin and History of Beer and Brewing is an exhaustive romp through thousands of years of beer history.

And this bust of Henius is in the Rebild National Park in Denmark. Henius organized fund-raising and “in 1911, almost 200 acres of the hilly countryside were bought with funds raised by Danish Americans. In 1912, Max Henius presented the deed to the land to his Majesty King Christian X as a permanent memorial to Danish Americans. Later the Danish government added to the land, that now features a beautiful natural park.”

And this is from the Chicago Midwest Rebild Chapter:

As we celebrate the 100th anniversary of the Rebild Society, I find it fascinating to look at the lives of its builders in the context of their times. It is hard to imagine a more dynamic time of porous borders and explosive growth than the late 19th century. Probably the name most closely associated with the founding of the Rebild Society is Max Henius. I had the good fortune to come across a biography of Henius written by his associates shortly after his death, and much of what I have written of Henius is largely based on that biography.

The first Europeans to come to Chicago were Pere Marquette and Louis Joliet in 1673 when they claimed Midwestern North America for Nouvelle France. Marquette and Joliet traveled up the Illinois River and portaged to the Chicago River and down to Lake Michigan. Joliet called for a canal to be built to connect the Illinois and Chicago rivers to stimulate trade and help France establish an economic empire in the New World. It was a prescient recommendation. Such a canal would indeed be built almost 200 years later, and an economic empire was ignited. Chicago would become the transport hub for a new nation, and not for New France.

In 1838, ten years before the canal was built connecting the Great Lakes and Mississippi watersheds, Max Henius’ father immigrated to Denmark from an impoverished Jewish family in Torun, Poland, traveling on foot to Aarhus, where his brother Jacob lived. The journey took six weeks. The elder Henius rose quickly in the distillery business first in Aarhus and then in København. He launched his own his distillery, Spritfabrikken in Aalborg in 1846, with money loaned from partners. In 1854 he returned to Torun to find a bride.

Born in 1859, Max was educated at the Aalborg Latin School and went on to study at the Polytechnic Institute in Hanover before matriculating at the University of Marburg, Germany. 1881 was a pivotal year for Max Henius. His father sold the distillery that Max had hoped to take over, and he had fallen in love with Johanne Heiberg. Both families disapproved of the relationship and Max Henius decided to immigrate to the US and subsequently send for his fiancée to come and marry him. Interestingly, a contemporary who would also become a very famous Danish-American, Jens Jensen, would immigrate to the US three years later partly because his prospective partner also did not meet family approval. A fellow student from Hannover and Warburg, Robert Wahl, told Max of the multiple opportunities available in the US, and later would partner with Henius in a very successful business.

Already in 1870 immigrants made up a larger proportion of the city’s population (48 percent) than any other place in North America. Chicago was quickly rebuilding after its massive destruction by fire in 1871 and Danish immigration was beginning to swell. Max Henius arrived in Chicago in October of 1881. Although he was a well educated and degreed chemist, his first jobs were as a door to door book salesman, errand boy for a pharmacy, and as a coal trimmer. Two years later he was employed by the Northern Pacific Railway to test the waters between Fargo, North Dakota and Bozeman, Montana but returned to Chicago to marry Johanne on June 4, 1883. With his savings he opened a drug store and subsequently formed Wahl & Henius, Analytical and Consulting Chemists with a lab at the back of the store. They established themselves as authorities on yeast culture and brewing.

Chicago was at this time one of the most rapidly growing cities in the world, the Shanghai of the late 19th century. Population growth was meteoric, fueled by decade after decade of immigration. But it was a wide open and divided city and hardly immune to the controversies of its time. May 1, 1886 saw a massive demonstration by workers (well advertised in the immigrant press) in favor of the eight-hour working day. Three days later the conflict culminated in a violent confrontation. The 1886 Haymarket Massacre took place in Chicago when an unknown person threw a dynamite bomb at police as they dispersed a public meeting. Chicago police fired on workers during a general strike for the eight-hour workday, killing several demonstrators and resulting in the deaths of several police officers. International Workers’ Day is the commemoration of the Haymarket Massacre. Ironically this would become a holiday officially celebrated throughout the Soviet bloc in the next century.

Henius did become involved in some of the public issues of this time. In 1892 a typhoid epidemic broke out in Chicago. Sewage was discharged into the Chicago River and subsequently found it way into Lake Michigan where Chicago’s water supply was tapped. Henius examined milk samples that were watered down and publicly spoke out on his findings. The waters of Lake Michigan were mapped bacteriologically so the water cribs were moved farther out in Lake Michigan.

Henius was very active in various Danish immigrant organizations, including the Danish-American Association, formed in Chicago in 1906. The idea for a Danish-American festival to be held in Denmark actually came from Ivar Kirkegaard, a Danish-American poet and editor. The first Danish-American rally was held in 1908 at Krabbesholm Folk High School on Skive Fjord. En route to the Krabbesholm festival, Henius was visiting Aarhus, when he learned of the planning for a national exposition to be held in Aarhus the following summer. He proposed to his fellow association members that they organize a Danish-American meeting for July 4, 1909. They filled an auditorium and persuaded the crown prince, later King Christian X, Georg Brandes and other noted Danes to speak at the event. Three years later Rebild Park was purchased by Danish Americans and set aside as a park, with the understanding that the site would be used to celebrate the 4th of July. Rebild Park was dedicated in 1912, and the first festival in Rebild was held on August 5, 1912.

Later Henius would found and head the Jacob A. Riis League of Patriotic Service to act as a clearing house for patriotic activities for Danish Americans during the First World War. The League grew out of a committee that managed the 3rd Liberty Load drive in Chicago among Danish-Americans. It also had among its objectives the preservation of Danish culture in America. Its influence was used with President Woodrow Wilson to include the question of the Danish border with Germany in the post war peace settlement. Henius would also be instrumental in establishing and supporting the Danes Worldwide Archives in Aalborg, initially housed in his childhood home of Sohngårdsholm.

Immigration has always been a controversial subject and resisted with varying degrees of success throughout history. But looking backwards one can only conclude it has been to our good fortune, and that our societies have been quite enriched and rejuvenated by the dynamism that immigrants have brought to us.

And Gary Gillman also has a nice overview of Henius’ life in a blog post last year, entitled Max Henius, Star of American Brewing Science.

Historic Beer Birthday: Max Delbrück

June 16, 2017 By Jay Brooks Leave a Comment

Today is the birthday of Max Emil Julius Delbrück (June 16, 1850-May 4, 1919). He was a German chemist who spent most of his career exploring the fermentation sciences.

His Wikipedia entry is short:

Delbrück was born in Bergen auf Rügen. He studied chemistry in Berlin and in Greifswald. In 1872 he was made assistant at the Academy of Trades in Berlin; in 1887 he was appointed instructor at the Agricultural College, and in 1899 was given a full professorship. The researches, carried out in part by Delbrück himself, in part under his guidance, resulted in technical contributions of the highest value to the fermentation industries. He was one of the editors of the Zeitschrift für Spiritusindustrie (1867), and of the Wochenschrift für Brauerei. He died in Berlin, aged 68.

And here’s his entry from Today in Science:

Max Emil Julius Delbrück was a German chemist who spent a forty-five year career leading development in the fermentation industry. He established a school for distillation workers, a glass factory for the manufacture of reliable apparatus and instruments, and an experimental distillery. Giving attention to the raw resources, he founded teaching and experimental institutions to improve cultivation of potatoes and hops. He researched physiology of yeast and application in the process of fermentation, production of pure cultures, and the action of enzymes. He started the journals Zeitschrift fur Spiritus-Industrie (1867) and Wochenschrift für Brauerei, for the alcohol and brewery industries, which he co-edited.

Over the years, I’ve found a few great Delbrück quotes:

“Yeast is a machine.”

— Max Delbrück, from an 1884 lecture

“With the sword of science and the armor of Practice, German beer will encircle the world.”

          — Max Delbrück, from an address about yeast and fermentation in the
               brewery, to the German Brewing Congress as Director of the Experimental
               and Teaching Institute for Brewing in Berlin, June 1884

Historic Beer Birthday: William S. Gossett

June 13, 2017 By Jay Brooks Leave a Comment

Today is the birthday of William Sealy Gosset (June 13, 1876–October 16, 1937). He “was an English statistician. He published under the pen name Student, and developed the Student’s t-distribution.” He also worked his entire career for Guinness Brewing, and was trained as a chemist, but it was his pioneering work in statstics, in which he was self-taught, that he is remembered today.

Here’s his biography, from Wikipedia:

Born in Canterbury, England to Agnes Sealy Vidal and Colonel Frederic Gosset, Gosset attended Winchester College before studying chemistry and mathematics at New College, Oxford. Upon graduating in 1899, he joined the brewery of Arthur Guinness & Son in Dublin, Ireland.

As an employee of Guinness, a progressive agro-chemical business, Gosset applied his statistical knowledge – both in the brewery and on the farm – to the selection of the best yielding varieties of barley. Gosset acquired that knowledge by study, by trial and error, and by spending two terms in 1906–1907 in the biometrical laboratory of Karl Pearson. Gosset and Pearson had a good relationship. Pearson helped Gosset with the mathematics of his papers, including the 1908 papers, but had little appreciation of their importance. The papers addressed the brewer’s concern with small samples; biometricians like Pearson, on the other hand, typically had hundreds of observations and saw no urgency in developing small-sample methods.

Another researcher at Guinness had previously published a paper containing trade secrets of the Guinness brewery. To prevent further disclosure of confidential information, Guinness prohibited its employees from publishing any papers regardless of the contained information. However, after pleading with the brewery and explaining that his mathematical and philosophical conclusions were of no possible practical use to competing brewers, he was allowed to publish them, but under a pseudonym (“Student”), to avoid difficulties with the rest of the staff. Thus his most noteworthy achievement is now called Student’s, rather than Gosset’s, t-distribution.

Gosset had almost all his papers including The probable error of a mean published in Pearson’s journal Biometrika under the pseudonym Student. It was, however, not Pearson but Ronald A. Fisher who appreciated the importance of Gosset’s small-sample work, after Gosset had written to him to say I am sending you a copy of Student’s Tables as you are the only man that’s ever likely to use them!. Fisher believed that Gosset had effected a “logical revolution”. Fisher introduced a new form of Student’s statistic, denoted t, in terms of which Gosset’s statistic was {\displaystyle z={\frac {t}{\sqrt {n-1}}}} z=\frac{t}{\sqrt{n-1}}. The t-form was adopted because it fit in with Fisher’s theory of degrees of freedom. Fisher was also responsible for applications of the t-distribution to regression analysis.

Although introduced by others, Studentized residuals are named in Student’s honour because, like the problem that led to Student’s t-distribution, the idea of adjusting for estimated standard deviations is central to that concept.

Gosset’s interest in the cultivation of barley led him to speculate that the design of experiments should aim not only at improving the average yield but also at breeding varieties whose yield was insensitive to variation in soil and climate, i.e. robust. This principle only appeared in the later thought of Ronald Fisher, and then in the work of Genichi Taguchi during the 1950s.

In 1935, Gosset left Dublin to take up the position of Head Brewer, in charge of the scientific side of production, at a new Guinness brewery at Park Royal in northwestern London. He died two years later in Beaconsfield, England, of a heart attack.

Gosset was a friend of both Pearson and Fisher, a noteworthy achievement, for each had a massive ego and a loathing for the other. He was a modest man who once cut short an admirer with the comment that “Fisher would have discovered it all anyway.”

And this biography is from the MacTutor History of Mathematics archive:

William Sealey Gosset was born on June 13, 1876 in Canterbury, England where he was the oldest of five children. He died at the age of 61 in Beaconsfield, England on October 16, 1937. He attended the Royal Military Academy in Woolwich to b ecome an engineer before he was rejected because of poor eyesight. William Gosset was never employed as a statistician. In a world of quarrelsome statistics, but he got along with everyone. He was a very helpful, quiet, patient and loyal person.

He went to school at Winchester and was well educated before entering the New College in Oxford. Here he won a first degree in chemistry in 1899. After getting his degree as a chemist, he got a job at Guinness brewery in Dublin in 1899, where he did important work on statistics, but her was never hired at a statistician. It was his environment at Guinness’ that made him a statistician. The brewery was interested in how they could make the best beer.

In 1900, the Guinness Research Laboratory was opened, which was head by the most distinguished brewing chemist, Horace Brown. Horace Brown along with the other brews were wondering how to get the raw materials for brewing beer at the cheapest but getting the best. There were many factors that they had to take into account such as varieties of barley and hops, what conditions of dying, cultivation and maturing factors.

After a few years of research, given that they were given a free hand to explore the conditions of brewing. This gave Gosset a chance to work as a statistician. He was able to take the data from the different examples of brewing to help find out which way was the best. As the young brewers work together, it seemed natural for them to take the data to Gosset to solve the numerical problems.

Gosset, in 1903, could calculate standard errors. In 1904 he wrote on the brewing of beer. This report lead to Karl Pearson consulting Gosset. Gosset met Pearson in July of 1905 when they had long talk together. Pearson, in an hour and a half, m ade Gosset understand the theory of standard errors. Gosset went back to the brewery and practiced those method for the next year. The meeting was also successful in which Pearson got Gosset to take up the study of the law of error.

Gosset wrote paper in his spare time under the name “Student.” His paper were on the probability of error of the mean and of the correlation coefficient for publication. Gosset even managed to run cooperative experiments with Hunter a nd Bennett at Ballinacurra, Buffin at Cambridge, and Beaven at Warminster in the testing of seeds against other seeds. Gosset also work with R.A. Fisher. The funny part is that Fisher did not get along Pearson, but Gosset studied under Pearson and also got along with Fisher.

To quickly recap William Gosset, he was born in 1876 and died in 1937. He did mathematical research for beer brewing, but had the problem working with only a small sample size. He work on the concept of probable errror of a mean. He also analysi sed an extended and broad range of problems such as the counting with a haemacytometer, probable error of a correlation coefficient, cereals, agronomy and the Lanarkshire milk experiment.

A very personal friend, McMullen, said this about Gosset, “he was a very kindly and tolerant and absolutely devoid malice. He rarely spoke about personal matters but when his opinion was well worth listening to and not in the least superficia l.”

Pricenomics has a good overview of Gossett’s contributions to mathematics and statistics, entitled The Guinness Brewer Who Revolutionized Statistics.

Historic Beer Birthday: Carl von Linde

June 11, 2017 By Jay Brooks Leave a Comment

Today is the birthday of Carl Paul Gottfried Linde (June 11, 1842–November 16, 1934). He “was a German scientist, engineer, and businessman. He discovered a refrigeration cycle and invented the first industrial-scale air separation and gas liquefaction processes. These breakthroughs laid the backbone for the 1913 Nobel Prize in Physics. Linde was a member of scientific and engineering associations, including being on the board of trustees of the Physikalisch-Technische Reichsanstalt and the Bavarian Academy of Sciences and Humanities. Linde was also the founder of what is now known as The Linde Group, the world’s largest industrial gases company, and ushered the creation of the supply chain of industrial gases as a profitable line of businesses. He was knighted in 1897 as Ritter von Linde.”

His importance to brewing, especially yo lager beers, is undeniable. His first refrigerating machines were built for breweries. This is situation prior to his inventions, from the University of Chicago:

Before the development of mechanical refrigeration technologies, brewers were reliant on ice harvested from lakes and ponds and stored in ice-houses. The invention of mechanical refrigeration machines provided commercial brewers with the technology necessary to keep beer for longer periods of time. Refrigeration technology was also used in special railroad boxcars, permitting brewers to ship their product over longer distances. One of the most successful early designs for a mechanical refrigeration system was invented by Carl von Linde (a professor at Munich Polytechnic School) and was an ammonia-based vapor-compression system.

One of the drawing from his first patent, in 1873.

This history of the development of Linde’s refrigeration machines is from a brochure prepared by his the company he founded, The Linde Group.

Initial contacts with breweries

After von Linde had published his ideas in 1870 and 1871 in the Polytechnic Association’s “Bavarian Industry and Trade Journal,” which he also edited, a development was set in motion that would determine the direction of the entire rest of his life. His articles on refrigeration technology had aroused the interest of brewers who had been looking for a reliable year-round method of refrigeration for the fermentation and storage of their beer. In the summer of 1871 an agreement was made between von Linde, Austrian brewer August Deiglmayr (Dreher Brewery) and Munich brewer Gabriel Sedlmayr to build a test machine according to Linde’s design at the Spaten Brewery. With their help, Linde’s ideas would be put into practice, so that a refrigeration unit could then be installed at the Dreher Brewery, the largest brewery in Austria, in the hot, humid city of Trieste (now part of Italy).

Building the first Linde ice machine

The construction plans were finally completed in January 1873 and the patent applied for. The Bavarian patent required, however, that the machine be in operation within one year. Therefore von Linde and Sedlmayr placed an order with Maschinenfabrik Augsburg that same month to build it. And with some effort they succeeded in starting operation by the important patent deadline in January 1874. Of course, the first machine did have its difficulties.

The main problem was that von Linde’s mercury seal did not work properly so that the methyl ether used as the refrigerant leaked out of the compressor. In Linde’s words, “This design was not a suitable solution for the requirements of practical use. So it seemed imperative to build a second machine.”

In order to finance it, von Linde assigned part of the patent rights to Sedlmayr, to locomotive builder Georg Krauss and to the director of Maschinenfabrik Augsburg, Heinrich von Buz. In return, they provided the funds needed for the development, building and testing of a new refrigeration machine.

Building the second refrigeration machine

With his student and assistant Friedrich Schipper, von Linde designed a new compressor, which had a significantly simpler and more effective seal. The sealing material used in the newly designed gland construction was glycerin and the more efficient ammonia was used as the refrigerant. The new machine weighed and cost only half as much as its predecessor.

In the spring of 1875 Linde ordered the new compressor from Maschinenfabrik Augsburg and submitted it for a Bavarian patent, which was awarded on March 25, 1876 for ten years. He received the German Reichspatent in August 1877.

“The very first trials with this second compressor yielded fully satisfactory results,” said von Linde, not without pride. The machine was sold to the Dreher Brewery in September 1876, erected under Schipper’s supervision and started up in spring 1877. It ran until 1908, providing refrigeration and dehumidification

Technical breakthrough

But despite this success, Linde created a third design immediately after the second machine was installed at Dreher, turning his attention to gas pumps, which were already widely used. This third, horizontal design proved to be the best cold vapor machine on the market in terms of its price/performance ratio and became the standard type of Linde compressor for decades to come. During the more than six-year development and experimentation phase, a reliable solution also had to be found for distributing the generated cold. After long trials, in executing an order for the Heineken Brewery in Rotterdam, von Linde developed a method of circulating cold saltwater brine in a pipe cooling system (natural convection cooling), which was installed on the ceiling of the refrigeration rooms.

And this inset is about the company’s “First customers and partners: brewers.”

In 1840, many continental European breweries switched to bottom fermented lager production (in contrast to the “English” top-fermented brown beers or ales) because the beer remained fresh longer and customers preferred the taste. The ice machine described by von Linde seemed ideal for achieving the required lower temperatures and to ensure precise cooling control. So it is no wonder that some major brewers showed great interest in this invention.

Gabriel Sedlmayr of the Munich Spaten Brewerey was willing to let von Linde experiment with an early refrigeration machine in his brewery in the early 1870s. The first unit functioned passably well, but was too large and had numerous flaws. The drawings submitted for the patent showed that Sedlmayr himself had a hand in the second version, which was significantly smaller in size and worked well. This unit was sold to the Trieste Dreher Brewery for air cooling.

With Sedlmayr as an intermediary, the Rotterdam Heineken Brewery under its director Feldmann ordered an ice machine in 1877 for ice production. In his collaboration with the Heineken Brewery, Linde developed “natural convection cooling” with a system of cooling pipes under the ceiling of the cellar. Feldmann in turn put von Linde in contact with J. C. Jacobsen, head of the Carlsberg Brewery in Copenhagen, who ordered a large refrigeration unit in 1878.

Karl Lang, technical adviser and supervisory board member of several Rhineland breweries, also played a significant role during the founding period of the “Gesellschaft für Linde’s Eismaschinen.” He introduced Linde to brewery director Gustav Jung, who not only ordered a refrigeration unit but also became, with Lang and banker Moritz von Hirsch, a shareholder and Supervisory Board member of the Linde Company.

The connection between the Linde Company and brewery directors was maintained to some extent over several generations. After the death of Karl Lang in 1894 his position as chairman of the Supervisory Board was taken over by Gustav Jung, followed by his son Adolf Jung in 1886. Carl Sedlmayr took over for his father Gabriel on the Supervisory Board and in 1915, the third generation of this family followed with Anton Sedlmayr. The Jung and Sedlmayr families held their Supervisory Board seats until after the Second World War.

Here’s Linde’s entry from the Oxford Companion to Beer, written by Horst Dornbush.

Linde, Carl von
was a 19th-century German engineer and one of the world’s major inventors of refrigeration technology. See refrigeration. Starting in the middle of the 18th century, many people before Linde had tinkered with artificial refrigeration contraptions, but Linde was the first to develop a practical refrigeration system that was specifically designed for keeping fermenting and maturing beer cool—in Linde’s case, Bavarian lagers—during the hot summer months. Linde was born in the village of Berndorf, in Franconia, in 1842, at a time when warm-weather brewing was strictly forbidden in his native Bavaria; no one was allowed to brew beer between Saint George’s Day (April 23) and Michael’s Day (September 29). This was to avoid warm fermentations, which provided ideal habitats for noxious airborne bacteria to proliferate and caused yeasts to produce undesirable fermentation flavors. Both made summer beers often unpalatable. Summer brewing prohibition had been in force since 1553 and was only lifted in 1850, by which time Bavarian brewers had learned to pack their fermentation cellars with ice they had laboriously harvested in the winter from frozen ponds and lakes. There had to be a better way to keep beer cold…and that was just the challenge for a budding mechanical engineering professor like Linde, who had joined Munich’s Technical University in 1868. See weihenstephan. The basic principle of refrigeration had been understood for centuries. Because cold is merely the absence of heat, to make things cold, one must withdraw heat. Compressing a medium generates heat; subsequently decompressing or evaporating it quickly absorbs heat from its environment. Devices based on this principle are now generally known as vapor-compression refrigeration systems; apply this to a fermenting or lagering vessel, and it becomes a beer-cooling system. For Linde, the next question was the choice of refrigerant. Initially he experimented with dimethyl ether but eventually settled on ammonia because of its rapid expansion (and thus cooling) properties. He called his invention an “ammonia cold machine.” Linde had received much of the funding for this development from the Spaten Brewery in Munich, which was also the first customer to install the new device—then still driven by dimethyl ether—in 1873. By 1879, Linde had quit his professorship and formed his own “Ice Machine Company,” which is still in operation today as Linde AG, headquartered in Wiesbaden, Germany. By 1890, Linde had sold 747 refrigeration units machines to various breweries and cold storage facilities. He continued to innovate and invented new devices most of his life, including equipment for liquefying air, and for the production of pure oxygen, nitrogen, and hydrogen. In 1897 he was knighted, and from then on could append the honorific “von” to his surname. He died a prosperous industrialist in Munich in 1934, at the age of 92, and today Linde AG is a leading gases and engineering company with almost 48,000 employees working in more than 100 countries worldwide. For all his many accomplishments, Linde’s pioneering work in artificial beer cooling technology is perhaps his most enduring legacy.

Historic Beer Birthday: Eduard Buchner

May 20, 2017 By Jay Brooks Leave a Comment

Today is the birthday of Eduard Buchner (May 20, 1860-August 13, 1917). Buchner was a German chemist and zymologist, and was awarded with Nobel Prize in Chemistry in 1907 for his work on fermentation.

This is a short biography from The Famous People:

Born into an educationally distinguished family, Buchner lost his father when he was barely eleven years old. His elder brother, Hans Buchner, helped him to get good education. However, financial crisis forced Eduard to give up his studies for a temporary phase and he spent this period working in preserving and canning factory. Later, he resumed his education under well-known scientists and very soon received his doctorate degree. He then began working on chemical fermentation. However, his experience at the canning factory did not really go waste. Many years later while working with his brother at the Hygiene Institute at Munich he remembered how juices were preserved by adding sugar to it and so to preserve the protein extract from the yeast cells, he added a concentrated doze of sucrose to it. What followed is history. Sugar in the presence of enzymes in the yeast broke into carbon dioxide and alcohol. Later he identified the enzyme as zymase. This chance discovery not only brought him Nobel Prize in Chemistry, but also brought about a revolution in the field of biochemistry.

Eduard Buchner is best remembered for his discovery of zymase, an enzyme mixture that promotes cell free fermentation. However, it was a chance discovery. He was then working in his brother’s laboratory in Munich trying to produce yeast cell free extracts, which the latter wanted to use in an application for immunology.

To preserve the protein in the yeast cells, Eduard Buchner added concentrated sucrose to it. Bubbles began to form soon enough. He realized that presence of enzymes in the yeast has broken down sugar into alcohol and carbon dioxide. Later, he identified this enzyme as zymase and showed that it can be extracted from yeast cells. This single discovery laid the foundation of modern biochemistry.

One of the most important aspects of his discovery proving that extracts from yeast cells could elicit fermentation is that it “contradicted a claim by Louis Pasteur that fermentation was an ‘expression of life’ and could occur only in living cells. Pasteur’s claim had put a decades-long brake on progress in fermentation research, according to an introductory speech at Buchner’s Nobel presentation. With Buchner’s results, “hitherto inaccessible territories have now been brought into the field of chemical research, and vast new prospects have been opened up to chemical science.”

In his studies, Buchner gathered liquid from crushed yeast cells. Then he demonstrated that components of the liquid, which he referred to as “zymases,” could independently produce alcohol in the presence of sugar. “Careful investigations have shown that the formation of carbon dioxide is accompanied by that of alcohol, and indeed in just the same proportions as in fermentation with live yeast,” Buchner noted in his Nobel speech.

This is a fuller biography from the Nobel Prize organization:

Eduard Buchner was born in Munich on May 20, 1860, the son of Dr. Ernst Buchner, Professor Extraordinary of Forensic Medicine and physician at the University, and Friederike née Martin.

He was originally destined for a commercial career but, after the early death of his father in 1872, his older brother Hans, ten years his senior, made it possible for him to take a more general education. He matriculated at the Grammar School in his birth-place and after a short period of study at the Munich Polytechnic in the chemical laboratory of E. Erlenmeyer senior, he started work in a preserve and canning factory, with which he later moved to Mombach on Mainz.

The problems of chemistry had greatly attracted him at the Polytechnic and in 1884 he turned afresh to new studies in pure science, mainly in chemistry with Adolf von Baeyer and in botany with Professor C. von Naegeli at the Botanic Institute, Munich.

It was at the latter, where he studied under the special supervision of his brother Hans (who later became well-known as a bacteriologist), that his first publication, Der Einfluss des Sauerstoffs auf Gärungen (The influence of oxygen on fermentations) saw the light in 1885. In the course of his research in organic chemistry he received special assistance and stimulation from T. Curtius and H. von Pechmann, who were assistants in the laboratory in those days.

The Lamont Scholarship awarded by the Philosophical Faculty for three years made it possible for him to continue his studies.

After one term in Erlangen in the laboratory of Otto Fischer, where meanwhile Curtius had been appointed director of the analytical department, he took his doctor’s degree in the University of Munich in 1888. The following year saw his appointment as Assistant Lecturer in the organic laboratory of A. von Baeyer, and in 1891 Lecturer at the University.

By means of a special monetary grant from von Baeyer, it was possible for Buchner to establish a small laboratory for the chemistry of fermentation and to give lectures and perform experiments on chemical fermentations. In 1893 the first experiments were made on the rupture of yeast cells; but because the Board of the Laboratory was of the opinion that “nothing will be achieved by this” – the grinding of the yeast cells had already been described during the past 40 years, which latter statement was confirmed by accurate study of the literature – the studies on the contents of yeast cells were set aside for three years.

In the autumn of 1893 Buchner took over the supervision of the analytical department in T. Curtius’ laboratory in the University of Kiel and established himself there, being granted the title of Professor in 1895.

In 1896 he was called as Professor Extraordinary for Analytical and Pharmaceutical Chemistry in the chemical laboratory of H. von Pechmann at the University of Tübingen.

During the autumn vacation in the same year his researches into the contents of the yeast cell were successfully recommenced in the Hygienic Institute in Munich, where his brother was on the Board of Directors. He was now able to work on a larger scale as the necessary facilities and funds were available.

On January 9, 1897, it was possible to send his first paper, Über alkoholische Gärung ohne Hefezellen (On alcoholic fermentation without yeast cells), to the editors of the Berichte der Deutschen Chemischen Gesellschaft.

In October, 1898, he was appointed to the Chair of General Chemistry in the Agricultural College in Berlin and he also held lectureships on agricultural chemistry and agricultural chemical experiments as well as on the fermentation questions of the sugar industry. In order to obtain adequate assistance for scientific research, and to be able to fully train his assistants himself, he became habilitated at the University of Berlin in 1900.

In 1909 he was transferred to the University of Breslau and from there, in 1911, to Würzburg. The results of Buchner’s discoveries on the alcoholic fermentation of sugar were set forth in the book Die Zymasegärung (Zymosis), 1903, in collaboration with his brother Professor Hans Buchner and Martin Hahn. He was awarded the Nobel Prize in 1907 for his biochemical investigations and his discovery of non-cellular fermentation.

Buchner married Lotte Stahl in 1900. When serving as a major in a field hospital at Folkschani in Roumania, he was wounded on August 3, 1917. Of these wounds received in action at the front, he died on the 13th of the same month.

Historic Beer Birthday: Emil Christian Hansen

May 8, 2017 By Jay Brooks Leave a Comment

Today is the birthday of Emil Christian Hansen (May 8, 1842-August 27, 1909). Hansen was a “Danish botanist who revolutionized beer-making through development of new ways to culture yeast. Born poor in Ribe, Denmark, he financed his education by writing novels. Though he never reached an M.Sc., in 1876, he received a gold medal for an essay on fungi, entitled “De danske Gjødningssvampe.” In 1879, he became superintendent of the Carlsberg breweries. In 1883, he successfully developed a cultivated yeast that revolutionized beer-making around the world, because Hansen by refusing to patent his method made it freely available to other brewers. He also proved there are different species of yeast. Hansen separated two species: Saccharomyces cerevisiae, an over-yeast (floating on the surface of the fermenting beer) and Saccharomyces carlsbergensis, an under-yeast (laying on the bottom of the liquid).

Here’s his entry from Encyclopedia Britannica:

Danish botanist who revolutionized the brewing industry by his discovery of a new method of cultivating pure strains of yeast.

Hansen, who began his working life as a journeyman house painter, received a Ph.D. in 1877 from the University of Copenhagen. Two years later he was appointed head of the physiology department at the Carlsberg Laboratory in Copenhagen, where he remained until his death. His research was concerned mainly with yeasts that convert carbohydrates to alcohol, and in 1888 he published an article that described his method for obtaining pure cultures of yeast. The yeast grown from these single strains was widely adopted in the bottom-fermentation brewing industries. Further investigations led him to the discovery of a number of species of yeast. He defined the characters of the different species and devised a system of classification. After further study he devised additional methods for the culture and isolation of certain species.

Emil Hansen as a young man.

This is how Carlsberg describes Hansen’s breakthrough in 1883:

The Carlsberg Laboratory made its first major scientific breakthrough when Dr. Emil Chr. Hansen developed a method for propagating pure yeast.

Fluctuations in the beer quality were not unknown at the time, but had until then been solved by thorough cleaning of all installations after suspension of production. If a brew failed, there was no use in pasteurising it; it had to be destroyed.

In 1883, the Old Carlsberg beer got infected with the beer disease and all efforts were made to find a solution to the problem.

Dr. Emil Chr. Hansen who joined the Carlsberg Laboratory in 1878 was examining the beer, and he found that it contained wild yeast. Through his studies and analyses, he discovered that only a few types of yeast (the pure yeast) are suitable for brewing, and he developed a technique to separate the pure yeast from the wild yeast cells. The problem had been solved, and the new Carlsberg yeast – Saccharomyces Carlsbergensis – was applied in the brewing process.

The propagating method revolutionised the brewing industry. Rather than to patent the process, Carlsberg published it with a detailed explanation so that anyone could build propagation equipment and use the method. Samples of the yeast – Saccharomyces Carlsbergensis – were sent to breweries around the world by request and young brewers came to Carlsberg to learn the skills.

This is the entry from Wikipedia on the history of Saccharomyces Carlsbergensis:

So-called bottom fermenting strains of brewing yeast were described as early as the 14th century in Nuremberg and have remained an indispensable part of both Franconian and Bavarian brewing culture in southern Germany through modern times. During the explosion of scientific mycological studies in the 19th century, the yeast responsible for producing these so-called “bottom fermentations” was finally given a taxonomical classification, Saccharomyces pastorianus, by the German Max Reess in 1870.

In 1883 the Dane Emil Hansen published the findings of his research at the Carlsberg brewery in Copenhagen and described the isolation of a favourable pure yeast culture that he labeled “Unterhefe Nr. I” (bottom-fermenting yeast no. 1), a culture that he identified as identical to the sample originally donated to Carlsberg in 1845 by the Spaten Brewery of Munich. This yeast soon went into industrial production in Copenhagen in 1884 as Carlberg yeast no. 1.

In 1904 Hansen published an important body of work where he reclassified the separate yeasts he worked with in terms of species, rather than as races or strains of the same species as he had previously done. Here Hansen classified a separate species of yeast isolated from the Carlsberg brewery as S. pastorianus, a name derived from and attributed to Reess 1870. This strain was admitted to the Centraalbureau voor Schimmelcultures (CBS) in 1935 as strain CBS 1538, Saccharomyces pastorianus Reess ex Hansen 1904. In a further publication in 1908, Hansen reclassified the original “Unterhefe Nr. I” as the new species Saccharomyces carlsbergensis and another yeast “Unterhefe Nr. II” as the new species Saccharomyces monacensis. The taxonomy was attributed to Hansen 1908 and the yeasts entered into the Centraalbureau voor Schimmelcultures in 1947 as CBS 1513 and CBS 1503 respectively.

Since the early 1900s, bottom-fermenting strains of brewery yeast have been typically classified as S. carlbergensis in scientific literature, and the earlier valid name assigned to a bottom-fermenting yeast by Reess in 1870 was rejected without merit. This situation was rectified using DNA-DNA reallocation techniques in 1985 when Vaughan-Martini & Kurtzman returned the species name to S. pastorianus under the type strain CBS 1538 and relegated the two former species assigned by Hansen in 1908, S. carlsbergensis CBS 1513 and S. monacensis CBS 1503, to the status of synonyms. These experiments also clearly revealed the hybrid nature of the lager brewing yeast species for the first time, even though one of the parental species was incorrectly classified in retrospect. Nonetheless, over the last decades of the 20th century, debate continued in scientific literature regarding the correct taxon, with authors using both names interchangeably to describe lager yeast.

Although most accounts mention that he wrote novels to put himself through school, one has a slightly different take, though I’m not sure how true it is. “Emil earned his bread and butter as a painter but he yearned for another life and left Ribe so he could study. He graduated from High School relatively late – he was 29 years old.”

Emil Christian Hansen, taken in 1908, a year before his death.

Historic Beer Birthday: William Cullen

April 15, 2017 By Jay Brooks Leave a Comment

Today is the birthday of William Cullen (April 15, 1710-February 5, 1790). He “was a Scottish physician, chemist and agriculturalist, and one of the most important professors at the Edinburgh Medical School, during its hay-day as the leading center of medical education in the English-speaking world.

Cullen was also a central figure in the Scottish Enlightenment. He was David Hume’s physician and friend, and on intimate terms with Adam Smith, Lord Kames (with whom he discussed theoretical and practical aspects of husbandry), Joseph Black, John Millar, and Adam Ferguson, among others.

He was President of the Royal College of Physicians and Surgeons of Glasgow (1746–47), President of the Royal College of Physicians of Edinburgh (1773–1775) and First Physician to the King in Scotland (1773–1790). He was also, incidentally, one of the prime movers in obtaining a royal charter for the Philosophical Society of Edinburgh, resulting in the formation of the Royal Society of Edinburgh in 1783.”

Cullen extended the subject of chemistry beyond medicine by connecting it to many “arts” including agriculture, bleaching, brewing, mining, and the manufacture of vinegar and alkalies. In brewing, it was the very important need for cooling using artificial refrigeration where William Cullen at the University of Glasgow in 1748 made his impact, making advances crucial to the development of refrigeration for the brewing industry when he began studying the cooling effects of liquids evaporating in a vacuum, the process by which we cool foods today. He even demonstrated artificial refrigeration for the first time in 1748.

In the Brussels Journal, in a multi-part history of beer, Cullen’s contributions are acknowledged and explained:

The principle of vacuum refrigerators is based on the fact that water in a sealed container can be made to boil if the pressure is reduced (the “boiling point” of 100 degrees Celsius refers to the situation when the external pressure equals one atmosphere; water can be made to boil at lower temperatures on a mountain top). The heat necessary for evaporation is taken from the water itself. Reducing the pressure further lowers the temperature until freezing-point is reached and ice is formed. The Scottish scholar and chemist William Cullen (1710-1790) gave one of the first documented public demonstrations of artificial refrigeration, and the United States inventor Oliver Evans (1755-1819) designed, but did not build, a refrigeration machine which ran on vapor in 1805. I. Hornsey writes in his history of beer and brewing:

“The earliest machine of this type was constructed in 1755, by Dr William Cullen, who produced the vacuum necessary purely by means of a pump. Then, in 1810, Sir John Leslie combined a vessel containing a strong sulphuric acid solution along with the air pump, the acid acting as an absorbent for water vapour in the air. This principle was taken up and elaborated upon by E.C. Carré, who in 1860 invented a machine that used ammonia as the volatile liquid instead of water….The first compression machine was manufactured by John Hague in 1834, from designs by the inventor, Jacob Perkins, who took out the original patents, and recommended that ether was used as the volatile agent. Although Hague’s machine can be regarded as the archetype for all ‘modern’ refrigerators, it never really got past the development stage, and it was left to the Australian, James Harrison, of Geelong, Victoria, to finalise the practicalities and produce a working version, which he did in 1856. By 1859, Harrison’s equipment was being manufactured commercially in New South Wales, and the first of them (which used ether as the refrigerating agent) came to Britain in 1861.”

Although the first inventor of a practical refrigerator was Oliver Evans in 1805, Cullen invented the process in 1748 which allowed the technology to be further developed. After his public demonstration of the refrigeration effects of evaporative cooling, he described the phenomenon in “Of the Cold Produced by Evaporating Fluids and of Some Other Means of Producing Cold” (Essays and Observations, Physical and Literary, vol. 2 [1756]).

Historic Beer Birthday: Joseph Priestley

March 13, 2017 By Jay Brooks Leave a Comment

Today is the birthday of English scientist Joseph Priestley (March 13, 1733-February 6, 1804). While he was also a “clergyman, natural philosopher, chemist, educator, and Liberal political theorist,” he’s perhaps best known for discovering oxygen (even though a few others lay claim to that honor). According to Wikipedia, “his early scientific interest was electricity, but he is remembered for his later work in chemistry, especially gases. He investigated the ‘fixed air’ (carbon dioxide) found in a layer above the liquid in beer brewery fermentation vats. Although known by different names at the time, he also discovered sulphur dioxide, ammonia, nitrogen oxides, carbon monoxide and silicon fluoride. Priestley is remembered for his invention of a way of making soda-water (1772), the pneumatic trough, and recognising that green plants in light released oxygen. His political opinions and support of the French Revolution, were unpopular. After his home and laboratory were set afire (1791), he sailed for America, arriving at New York on 4 Jun 1794

In the biography of Priestley at the American Chemistry Society has a sidebar about his work with fermentation:

Bubbling Beverages

In 1767, Priestley was offered a ministry in Leeds, Englane, located near a brewery. This abundant and convenient source of “fixed air” — what we now know as carbon dioxide — from fermentation sparked his lifetime investigation into the chemistry of gases. He found a way to produce artificially what occurred naturally in beer and champagne: water containing the effervescence of carbon dioxide. The method earned the Royal Society’s coveted Copley Prize and was the precursor of the modern soft-drink industry.

Even Michael Jackson, in 1994, wrote about Priestley connection to the brewing industry.

“It has been suggested that the Yorkshire square system was developed with the help of Joseph Priestley who, in 1722, delivered a paper to the Royal Society on the absorption of gases in liquids. In addition to being a scientist, and later a political dissident, he was for a time the minister of a Unitarian church in Leeds. During that period he lived next to a brewery on a site that is now Tetley’s.”

In the New World Encyclopedia, during his time in Leeds, it explains his work on carbonation.

Priestley’s house was next to a brewery, and he became fascinated with the layer of dense gas that hung over the giant vats of fermenting beer. His first experiments showed that the gas would extinguish lighted wood chips. He then noticed that the gas appeared to be heavier than normal air, as it remained in the vats and did not mix with the air in the room. The distinctive gas, which Priestley called “fixed air,” had already been discovered and named “mephitic air” by Joseph Black. It was, in fact, carbon dioxide. Priestley discovered a method of impregnating water with the carbon dioxide by placing a bowl of water above a vat of fermenting beer. The carbon dioxide soon became dissolved in the water to produce soda water, and Priestley found that the impregnated water developed a pleasant acidic taste. In 1773, he published an article on the carbonation of water (soda water), which won him the Royal Society’s Copley Medal and brought much attention to his scientific work.

He began to offer the treated water to friends as a refreshing drink. In 1772, Priestley published a paper entitled Impregnating Water with Fixed Air, in which he described a process of dripping sulfuric acid (or oil of vitriol as Priestley knew it) onto chalk to produce carbon dioxide and forcing the gas to dissolve by agitating a bowl of water in contact with the gas.

And here’s More About Priestley from the Birmingham Jewellry Quarter, whatever that is:

But his most important work was to be in the field of gases, which he called ‘airs’ (he would later chide James Keir for giving himself airs (oh dear!) by adopting the term ‘gases’ in his Dictionary of Chemistry, saying ‘I cannot help smiling at his new phraseology’). Living, as he did at the time, next to a brewery, he noticed that the gas given off from the fermenting vats drifted to the ground, implying that it was heavier than air. Moreover, he discovered that it extinguished lighted wood chips. He had discovered carbon dioxide, which he called ‘fixed air’. Devising a method of making the gas at home without brewing beer, he discovered that it produced a pleasant tangy taste when dissolved in water. By this invention of carbonated water, he had become the father of fizzy drinks!

But perhaps my favorite retelling comes from the riveting History of Industrial Gases:

The relevant findings were published in 1772, in Impregnating Water with Fixed Air

20. By this process may fixed air be given to wine, beer, and almost any liquor whatever: and when beer is become flat or dead, it will be revived by this means; but the delicate agreeable flavour, or acidulous taste communicated by the fixed air, and which is manifest in water, will hardly be perceived in wine, or other liquors which have much taste of their own.

Priestley’s apparatus for experimenting with ‘airs.’

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