Historic Beer Birthday: Louis Pasteur

December 27, 2024 By Jay Brooks

Today is the birthday of Louis Pasteur (December 27, 1822–September 28, 1895). He “was a French chemist and microbiologist renowned for his discoveries of the principles of vaccination, microbial fermentation and pasteurization. He is remembered for his remarkable breakthroughs in the causes and preventions of diseases, and his discoveries have saved countless lives ever since. He reduced mortality from puerperal fever, and created the first vaccines for rabies and anthrax. His medical discoveries provided direct support for the germ theory of disease and its application in clinical medicine. He is best known to the general public for his invention of the technique of treating milk and wine to stop bacterial contamination, a process now called pasteurization. He is regarded as one of the three main founders of bacteriology, together with Ferdinand Cohn and Robert Koch, and is popularly known as the ‘father of microbiology.'”

Portrait of Louis Pasteur

But, of course, for the brewing industry, he’s best remembered for his “Studies on Fermentation,” which he published in 1876.

In 1876, Louis Pasteur published his ground-breaking volume, Études sur la Bière, soon translated into English as Studies On Fermentation. The book changed the course of brewing during the late 19th and early 20th centuries, representing a huge leap forward in the scientific understanding of the processes involved in beermaking. Brewers around the globe put Pasteur’s findings to work in their breweries, and thus plunged the industry headlong into the modern era.

In tribute to Pasteur’s tremendous contributions to brewing science, BeerBooks.com has reprinted Studies On Fermentation exactly as it appeared when first released in English, complete with all of Pasteur’s illustrations. An original 1879 edition was digitally scanned, professionally enhanced and reproduced in a hard cover format.

In his preface, Pasteur modestly wrote, “I need not hazard any prediction concerning the advantages likely to accrue to the brewing industry from the adoption of such a process of brewing as my study of the subject has enabled me to devise, and from an application of the novel facts upon which this process is founded. Time is the best appraiser of scientific work, and I am not unaware that an industrial discovery rarely produces all its fruits in the hands of its first inventor.”

But, of course, the brewing industry recognized almost immediately the impact that Pasteur’s work would have on the art and science of beermaking. Frank Faulkner, the British brewing scholar who performed the English translation, wrote, “Seeing the vast importance of Pasteur’s work from a practical point of view, after writing a review of it for the Brewers’ Journal, I determined to procure, at any rate for the use of my own pupils, a literal translation, illustrated by photo-lithographic copies of the original plates…It was on the completion of this translation that my views and desires expanded. The more I studied the work, the more I was convinced of its immense value to the brewer as affording him an intelligent knowledge of the processes and materials with which he deals…I determined accordingly to publish the work if I could secure the consent of its distinguished author…The debt which we English brewers owe to M[r]. Pasteur can hardly be over-estimated.”

While I’m sure there are probably many more, he had patents I highlighted last year, Patent No. 135245A: Improvement in Brewing Beer and Ale from 1873, and Patent No. 141072A: Manufacture of Beer and Yeast, in the same year.

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A portrait of Louis Pasteur painted by Swedish-speaking Finnish artist Albert Edelfelt, in 1886.

Fermentation and germ theory of diseases

Pasteur demonstrated that fermentation is caused by the growth of micro-organisms, and the emergent growth of bacteria in nutrient broths is due not to spontaneous generation, but rather to biogenesis (Omne vivum ex vivo “all life from life”). He was motivated to investigate the matter while working at Lille. In 1856 a local wine manufacturer, M. Bigot, the father of his student, sought for his advice on the problems of making beetroot alcohol and souring after long storage. In 1857 he developed his ideas stating that: “I intend to establish that, just as there is an alcoholic ferment, the yeast of beer, which is found everywhere that sugar is decomposed into alcohol and carbonic acid, so also there is a particular ferment, a lactic yeast, always present when sugar becomes lactic acid.” According to his son-in-law, Pasteur presented his experiment on sour milk titled “Latate Fermentation” in August 1857 before the Société des Sciences de Lille. (But according to a memoire subsequently published, it was dated November 30, 1857). It was published in full form in 1858. He demonstrated that yeast was responsible for fermentation to produce alcohol from sugar, and that air (oxygen) was not required. He also demonstrated that fermentation could also produce lactic acid (due to bacterial contamination), which makes wines sour. This is regarded as the foundation of Pasteur’s fermentation experiment and disprove of spontaneous generation of life.

Pasteur’s research also showed that the growth of micro-organisms was responsible for spoiling beverages, such as beer, wine and milk. With this established, he invented a process in which liquids such as milk were heated to a temperature between 60 and 100 °C. This killed most bacteria and moulds already present within them. Pasteur and Claude Bernard completed the first test on April 20, 1862. Pasteur patented the process, to fight the “diseases” of wine, in 1865. The method became known as pasteurization, and was soon applied to beer and milk.

Beverage contamination led Pasteur to the idea that micro-organisms infecting animals and humans cause disease. He proposed preventing the entry of micro-organisms into the human body, leading Joseph Lister to develop antiseptic methods in surgery. Lister’s work in turn inspired Joseph Lawrence to develop his own alcohol-based antiseptic, which he named in tribute Listerine.

This is an ad is for Whitbread, from 1937, showing an illustration of Louis Pasteur working on his fermentation studies in a laboratory at Whitbread Brewing in 1871, nine years after he completed his first test of pasteurization, which took place April 20, 1862.

On his Wikipedia page, under the heading “Controversies, there’s this paragraph about his research into fermentation:

When Pasteur published his theory and experiments on fermentation in 1858, it was not new to science, neither the idea nor the experiment. In 1840 a German chemist Justus von Liebig had noted that yeast could induce fermentation in water. However, he did not know that yeasts were organisms. In 1856 another German, Friedrich Wilhelm Lüdersdorff, reported that yeasts were microorganisms that convert sugar into alcohol. In 1855, Antoine Béchamp, Professor of Chemistry at the University of Montpellier, showed that sugar was converted to sucrose and fructose in a closed bottle containing water and when he added calcium or zinc chloride to it, no reaction occurred. He also noticed moulds developing in the solution, but could not fathom the significance of it. He concluded that water was the factor for fermentation. He changed his conclusion in 1858 that water was not the main factor, in fact, fermentation was directly related to the growth of moulds, and moulds required air for growth. He regarded himself as the first to show the role of microorganisms in fermentation. Pasteur started his experiments only in 1857 and published his findings in 1858 (April issue of Comptes Rendus Chimie, Béchamp’s paper appeared in January issue), which, as Béchamp noted, did not bring any novel idea or experiments that earlier works had not shown. On the other hand, Béchamp was probably aware of Pasteur’s 1857 preliminary works. With both scientists claiming priority on the discovery, a bitter and protracted dispute lasted throughout their lives. Their rivalry extended to ideas on microbiology, pathogenesis, and germ theory. Particularly on the spontaneous generation because Pasteur in his 1858 paper explicitly stated that the lactic acid bacteria (he named them “lactic yeasts”), which caused wine souring, “takes birth spontaneously, as easily as beer yeast every time that the conditions are favourable.” This statement directly implied that Pasteur did believe in spontaneous generation. He condemned the ideas of Pasteur as “‘the greatest scientific silliness of the age”. However, Béchamp was on the losing side, as the BMJ obituary remarked: His name was associated with bygone controversies as to priority which it would be unprofitable to recall. Pasteur and Béchamp believed that fermentation was exclusively cellular activity, that is, it was only due to living cells. But later extraction of enzymes such as invertase by Marcellin Berthelot in 1860 showed that it was simply an enzymatic reaction.

Pasteur’s ground-breaking “Studies on Fermentation” is in the public domain, of course, so you can read the entire work, or just browse through it, at the Internet Archive.

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In 1951, the United States Brewers Foundation featured Louis Pasteur in an ad, which was part of a series that used a Q&A format aimed at highlighting different positive aspects of beer and the brewing industry.

Historic Beer Birthday: Gerardus Johannes Mulder

December 27, 2024 By Jay Brooks

Today is the birthday of Gerardus Johannes Mulder (December 26, 1802–April 18, 1880). He “was a Dutch organic and analytical chemist,” who wrote several technical chemical publications analyzing various substances, including one entitled “The Chemistry of Beer,” in 1856 or 57 (sources vary).

“He became a professor of chemistry at Rotterdam and later at Utrecht. While at the Utrecht University, Mulder described the chemical composition of protein. He claimed that albuminous substances are made up of a common radical, protein, and that protein had the same empirical formula except for some variation in amounts of sulfur and phosphorus, long before the polymer nature of proteins was recognized after work by Staudinger and Carrothers.

He was the first to use this name, protein, coined by Jöns Jacob Berzelius in a publication, his 1838 paper ‘On the composition of some animal substances’ (originally in French but translated in 1839 to German). In the same publication he also proposed that animals draw most of their protein from plants.”

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Here’s a biography of Mulder from Encyclopedia.com:

Mulder studied medicine at the University of Utrecht (1819–1825), from which he graduated with a dissertation on the action of alkaloids of opium, De opio ejusque principiis, actione inter se comparatis (1825). He practiced medicine in Amsterdam and then in Rotterdam, where he also lectured at the Bataafsch Genootschap der Proefondervindelijke Wijsbegeerte and taught botany to student apothecaries. At the foundation of a medical school at Rotterdam (1828), Mulder became lecturer in botany, chemistry, mathematics, and pharmacy. His attention was directed primarily to the practical training of his students. In 1840 Mulder succeeded N. C. de Fremery as professor of chemistry at the University of Utrecht. He applied for his retirement in 1868 and spent the rest of his life in Bennekom. Besides publishing on scientific subjects, Mulder took an active part in education, politics and public health. The works of Faraday and Berzelius exerted a great influence on him; his Leerboek voor Scheikundige werktuigkunde (1832–1835) was written in the spirit of Faraday’s Chemical Manipulation. Mulder edited a Dutch translation by three of his students of Berzelius’ textbook of chemistry as Leerboek der Scheikunde (6 vols., 1834–1845). His difficult character caused problems with some of his pupils and with other chemists.

From 1826 to 1865 Mulder edited five Dutch chemical journals (see bibliography), in which most of his work was published. He worked in physics and in both general and physical chemistry, the latter in combination with medicine, physiology, agriculture, and technology. Most of his work had a polemic character. His most important contributions are in the field of physiological chemistry and soil chemistry, in which he published two extensive works that attracted much attention in translation despite their many mistakes and erroneous speculations.

Studies on proteins led Mulder to his protein theory (1838): he supposed that all albuminous substances consist of a radical compound (protein) of carbon, hydrogen, nitrogen, and oxygen, in combination with varying amounts of sulfur and phosphorus. The differences among proteins resulted from multiplication of the protein units in conjunction with the two other elements. Thus, casein was formulated as

10 protein units + S,

And serum albumin as

10 protein units + SP2.

In 1843 Mulder published the first volume of a treatise on physiological chemistry, which was translated into English as The Chemistry of Vegetable and Animal Physiology (1845–1849). At first both Liebig and Berzelius accepted Mulder’s analysis of proteins; but Liebig soon opposed the theory vigorously, and a deep conflict with Mulder ensued. In 1839–1840 Mulder investigated humic and ulmic acids and humus substances and determined the amounts of geic acid (acidum geïcum), apocrenic acid (acidum apocrenicum or Quellsatzsäure), crenic acid (acidum crenicum or Quellsäure), and humic acids in fertile soils (1844). The structure of these various brown or black substances is unknown. They are a group of aromatic acids of high molecular weight, which can be extracted from peat, turf, and decaying vegetable matter in the soil. The difference between these acids is the oxygen content. In the decay of vegetable matter ulmic acid is formed. According to Mulder, this has the formula C20H14O6(in modern equivalents). In contact with air and water more oxygen is absorbed, which results in the successive formation of humic acid (C20H12O6, geic acid (C20H12O7), apocrenic acid C24H12O12, and crenic acid (C12H12O8).

His studies on agricultural chemistry led to the treatise De scheikunde der bouwbare aarde (1860). Mulder confirmed Berzelius’ suggestion that theine and caffeine are identical (1838) and was the first to analyze phytol correctly in his researches on chlorophyll. Among his other works are technical chemical publications on indigo (1833), wine (1855), and beer (1856), detailed research on the assaying method for analyzing silver in relation to the volumetric silver determination of Gay-Lussac (1857), and a study on drying oils (1865).

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While I can’t find the book itself, Julius E. Thausing mentions it in his “Theory and Practice of the Preparation of Malt and the Fabrication of Beer,”published in 1882.

Historic Beer Birthday: Michael Edward Ash

December 17, 2024 By Jay Brooks

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Today is the birthday of Michael Edward Ash (December 17, 1927–April 30, 2016). He “was a British mathematician and brewer. Ash led a team that invented a nitrogenated dispense system for Guinness stout, which evolved to become the beer now sold globally as Draught Guinness. As the manager in charge of the Easy Serve project, Ash is credited as the inventor of nitrogenated beer (sometimes known as “nitro beer” colloquially).”

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Michael Ash in the 1950s.

“Following graduation from Cambridge, Ash lectured in mathematics at The Bedford College for women for three years before joining Guinness & Co. at their London Brewery in Park Royal in January 1951.

After training as a brewer by 1954 Ash also had experience of running two departments (Brewing and Forwarding) and in 1955 he was given his own department the ‘Sample Room’, which had facilities for experimentation. The ‘Draught problem’ was given to Ash as part of his briefing from the managing Director, Hugh Beaver. At the time, Guinness used a convoluted draught system in which highly conditioned beer was blended with aged, nearly still beer. It was a slow, arduous process that limited the ability of draught dispense to reach a more global market.

Guinness had for years been looking for a system in which a barman with no special training could pour a glass of draught in a matter of seconds to settle quickly with a head (3/8″ in a normal ½ pint glass).

Ash realized the solution lay in the use of a blend of nitrogen and carbon dioxide (beer typically just uses the latter), but it took years to figure out a mechanism to dispense nitrogenated beer. Inside Guinness, Ash’s quest was regarded as quixotic, and other brewers chided it as “daft Guinness” and the “Ash Can.” Eventually, working with a keg designer, Ash hit on a revolutionary, self-contained two-part keg, with one chamber full of beer and the other full of mixed gas under pressure, and the introduction of nitrogen.[5] Nitrogen is less soluble than carbon dioxide, which allows the beer to be put under high pressure without making it fizzy. The high pressure of dissolved gas is required to enable very small bubbles to be formed by forcing the draught beer through fine holes in a plate in the tap, which causes the characteristic ‘surge’.

Ultimately called the “easy serve system,” it began to replace the old “high and low” taps used in Ireland and spread to Great Britain and beyond beginning in the 1960s. The invention, which made for a smoother, less characterful beer, was not without controversy, and for years a minority of Irish drinkers complained about the change. Eventually, nitrogrenated stout became a standard, not just at Guinness but among all Irish makers of stout.

Ash left the brewing side of Guinness in 1962 to become managing director of Crooks Laboratories in Park Royal (owned by Guinness). Crooks moved to Basingstoke in 1965. At Crooks Ash was responsible for acquiring the licence for the anti-depressant Prothiaden (Dosulepin) in 1967. From 1970 onwards Ash followed various interests including business education and was a founding governor of Templeton College Oxford.”

Ash with Pete Brown.Pete Brown, who’d met Ash recently, wrote his obituary for the Morning Advertiser after he passed away in April of this year at 88 years old, entitled The man who created the nitro stout.

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A photograph of Ash taken by Jeff Alworth during his visit to Guinness in early 2016.

Similarly, Jeff Alworth wrote a piece for All About Beer Magazine, The Man Who Invented Nitro the month after he passed away.

Alworth’s article online includes an audio clip of Michael Ash describing the process he used to create Draught Guinness using nitrogen.

And this biography of Ash was prepared by Guinness’ marketing department:

Michael read mathematics at Trinity College, Cambridge and was awarded a triple first in his studies – top scholar that year in Cambridge. Between 1948 – 1950, Michael was allowed to reduce his national service conscription by teaching Maths at a University (other than Oxbridge). He taught at Bedford College. Up to the end of World War Two, the Guinness Company had a policy of recruiting only first class honours science graduates from Oxford or Cambridge. Michael was the first non-brewer to be recruited into Guinness.

It was in this role, he led a team of over 20, and their primary role was to seek to improve the shelf life of bottled Guinness. However, Michael felt that the real prize was in developing a proper system for Draught Guinness and began dedicating his time to the ‘Draught Problem’.

The rise of lagers available on draught, especially in the UK in the 1940s and 1950s, was encroaching on traditional Guinness sales, and Michael felt that there was a great opportunity for Guinness, should the stout be available in Draught format. However, the essential problem was with the gas. Carbon dioxide was used to pressurise kegs of bitter and lager, and it was easy and effective for everyone concerned. Guinness, though was too lively to be draughted with carbon dioxide alone.

Of the 20 plus men on his Sample Room team, he could only afford to assign 2 people to work part-time with him on ‘Daft Guinness’ as it became known with the Park Royal Brewery. Michael talks about working weekends and late nights over a long period of time to eventually come up with a nitrogen gas solution.

He worked hand in hand with Eric Lewis, of Alumasc, who supplied Michael with prototype after protoype of metal kegs with different experimental gas chambers. The fact that nitrogen is an inert gas meant that they bubbles lasted longer and were smaller. The right amount of nitrogen, created the ‘surge’ and allowed for a controlled, creamy head that lasts for the whole pint.

The eventual solution was a ‘mixed gas dispense’ system. Known initially as ‘The Ash Can’, The Easy Serve Cask was a self-contained, two-part keg, with one chamber full of beer and the other full of mixed gas under pressure.

Having seen the possibilities, [the company] was in a hurry to get Draught Guinness out into the market place, and he demanded that it should be launched in 1959 – the year of the Guinness bicentenary. At a board meeting of 9 December 1959 – Viscount Elveden (later 3rd Lord Iveagh) reported that about half the draught Guinness outlets had now been changed to the Easy Serve system, and the changeover of the remainder should take place by mid-January 1960.

Here’s a short video that Guinness made about Michael Ash. Unfortunately, apparently you can’t even WATCH a video about beer unless you’re old enough, whiuch is maddneingly stupid, so go watch it on YouTube.

Historic Beer Birthday: William Painter

November 20, 2024 By Jay Brooks

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Today is the birthday of William Painter (November 20, 1838-July 15, 1906). He was born in Ireland, and in 1858 came to the U.S. “in search of better opportunities,” and settled in Baltimore, Maryland. He trained as a mechanical engineer and initially got a job “as a foreman at the Murrill & Keizer’s machine shop.” His biggest claim to fame is that he “invented the crown cork bottle cap and bottle opener. He worked with manufacturers to develop a universal neck for all glass bottles and started Crown Cork and Seal in 1892 to manufacture caps that could be used to seal the universal necks.”

William Painter and his father, Dr. Edward Painter : sketches and reminiscences

Over the course of his life, “Painter patented 85 inventions, including the common bottle cap, the bottle opener, a machine for crowning bottles, a paper-folding machine, a safety ejection seat for passenger trains, and a machine for detecting counterfeit currency. He was inducted to the National Inventors Hall of Fame in 2006.”

The bottle cap was arguably his most important invention. “The crown cork was patented by William Painter on February 2, 1892 (U.S. Patent 468,258). It had 24 teeth and a cork seal with a paper backing to prevent contact between the contents and the metal cap. The current version has 21 teeth. To open these bottles, a bottle opener is generally used.

The height of the crown cap was reduced and specified in the German standard DIN 6099 in the 1960s. This also defined the “twist-off” crown cap, now used in the United States, Canada, and Australia. This cap is pressed around screw threads instead of a flange, and can be removed by twisting the cap by hand, eliminating the need for an opener.”

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He also patented several other innovations for the brewing industry, such as the Bottle Seal Or Stopper, from 1894, the Bottle Stopper, in 1885, a Closure For Sealing Bottles, in 1899, and a Capped-Bottle Opener, from 1894, to name just a few.

And here’s Painter’s obituary from the Brewers Journal in 1906:

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Beer Birthday: Alfred Haunold

October 7, 2024 By Jay Brooks

Today is the birthday of Alfred Haunold (October 7, 1929- ). He was born in Hollabrunn, Austria and emigrated to the U.S. in the mid-1950s, eventually settling in Oregon. He worked as hop breeder for the U.S. Department of Agriculture and was in charge of a hop-breeding program in Corvallis, Oregon that was a partnership between Oregon State University and the USDA for over thirty years before he retired. He was responsible for Cascade, Willamette, Sterling, Liberty, and Mt. Hood, among at least eighteen additional hop varieties, not to mention his many other contributions to hop sciences.

Gary Gilman has the best summary of Dr. Haunold’s life and work with hops in an article on his Beer et seq. blog entitled Dr. Al Haunold — Craft Beer Pioneer.

He arrived from the East Coast to work on the problem of downy mildew in the Cluster hop, then a workhorse of U.S. brewing, as was the Oregon Fuggle, both primarily used for bittering beer. Aroma in beer, at the time, was the preserve of fine imported varieties, at least for premium beers. Hops such as the German Hallertau and Tettnang, Czech Saaz, and various English hops.

Haunold was an Austrian immigrant who had grown up on a farm about 60 miles from Vienna. He joined the USDA after doctoral studies in Nebraska, adding to his extensive Austrian qualifications.

Oregon State also recorded some interviews with him as a part of their Oral History Online program. Check out Al Haunold Oral History Interview #1, from November 18, 2014 and Al Haunold Oral History Interview #2, from August 1, 2017. He also sat down for two audio recordings in 1982, which you can find at the Oregon Hops & Brewing Archives. These resources are great if you want to hear firsthand accounts of the history of craft beer and the hops that made so many modern beers possible. ANd here’s a list of some of his research.

During his career, he was a Member American Society Brewing Chemists (member editorial board 1987-1995, chairman publication committee 1989-1993, board directors 1989-1993), Crop Science Society of America, and the Hop Research Council.

Historic Beer Birthday: John Gorrie

October 3, 2024 By Jay Brooks

Today is the birthday of John Gorrie (October 3, 1803-June 29, 1855). He “was a physician, scientist, inventor, and humanitarian,” and most importantly, is credited with creating one of the very first refrigerators, an important development for the brewing industry.

Here’s one brief account, from a history of refrigeration on The Sun:

The man credited with developing the first actual “fridge” was an American doctor, John Gorrie, who built an ice-maker in 1844 based on Evans’ work of decades earlier. He also pioneered air conditioning at the same time, since his idea was to blow air across the ice-making machine to cool hospital patients suffering from malaria in Florida.

Gorrie did not make the fortune he deserved. His business partner died and his leaky machines were mocked by the Press and the ice-producing firms to whom he could have been a threat. He died sick and broke aged 51.

Here’s his story from his Wikipedia page:

Since it was necessary to transport ice by boat from the northern lakes, Gorrie experimented with making artificial ice.

After 1845, he gave up his medical practice to pursue refrigeration products. On May 6, 1851, Gorrie was granted Patent No. 8080 for a machine to make ice. The original model of this machine and the scientific articles he wrote are at the Smithsonian Institution. In 1835, patents for “Apparatus and means for producing ice and in cooling fluids” had been granted in England and Scotland to American-born inventor Jacob Perkins, who became known as “the father of the refrigerator.” Impoverished, Gorrie sought to raise money to manufacture his machine, but the venture failed when his partner died. Humiliated by criticism, financially ruined, and his health broken, Gorrie died in seclusion on June 29, 1855. He is buried in Magnolia Cemetery.

Another version of Gorrie’s “cooling system” was used when President James A. Garfield was dying in 1881. Naval engineers built a box filled with cloths that had been soaked in melted ice water. Then by allowing hot air to blow on the cloths it decreased the room temperature by 20 degrees Fahrenheit. The problem with this method was essentially the same problem Gorrie had. It required an enormous amount of ice to keep the room cooled continuously. Yet it was an important event in the history of air conditioning. It proved that Dr. Gorrie had the right idea, but was unable to capitalize on it. The first practical refrigeration system in 1854, patented in 1855, was built by James Harrison in Geelong, Australia.

Schematic of Gorrie’s ice machine.And this account, entitled “Dr. John Gorrie, Refrigeration Pioneer,” is by George L. Chapel of the Apalachicola Area Historical Society in Apalachicola, Florida, which is the location of the John Gorrie State Museum:

Dr. John Gorrie (1803 – 1855), an early pioneer in the invention of the artificial manufacture of ice, refrigeration, and air conditioning, was granted the first U.S. Patent for mechanical refrigeration in 1851. Dr. Gorrie’s basic principle is the one most often used in refrigeration today; namely, cooling caused by the rapid expansion of gases. Using two double acting force pumps he first condensed and then rarified air. His apparatus, initially designed to treat yellow fever patients, reduced the temperature of compressed air by interjecting a small amount of water into it. The compressed air was submerged in coils surrounded by a circulating bath of cooling water. He then allowed the interjected water to condense out in a holding tank, and released or rarified, the compressed air into a tank of lower pressure containing brine; This lowered the temperature of the brine to 26 degrees F. or below, and immersing drip-fed, brick-sized, oil coated metal containers of non-saline water, or rain water, into the brine, manufactured ice bricks. The cold air was released in an open system into the atmosphere.

The first known artificial refrigeration was scientifically demonstrated by William Cullen in a laboratory performance at the University of Glasgow in 1748, when he let ethyl ether boil into a vacuum. In 1805, Oliver Evans in the United States designed but never attempted to build, a refrigeration machine that used vapor instead of liquid. Using Evans’ refrigeration concept, Jacob Perkins of the U.S. and England, developed an experimental volatile liquid, closed-cycle compressor in 1834.

Commercial refrigeration is believed to have been initiated by an American businessman, Alexander C. Twinning using sulphuric ether in 1856. Shortly afterward, an Australian, James Harrison, examined the refrigerators used by Gorrie and Twinning, and introduced vapor (ether) compression refrigeration to the brewing and meat packing industries.

The granting of a U.S. Patent in 1860 to Ferdinand P.E. Carre of France, for his development of a closed, ammonia-absorption system, laid the foundation for widespread modern refrigeration. Unlike vapor-compression machines which used air, Carre used rapidly expanding ammonia which liquifies at a much lower temperature than water, and is thus able to absorb more heat. Carre’s refrigeration became, and still is, the most widely used method of cooling. The development of a number of synthetic refrigerants in the 1920’s, removed the need to be concerned about the toxic danger and odor of ammonia leaks.

The remaining problem for the development of modern air conditioning would not be that of lowering temperature by mechanical means, but that of controlling humidity. Although David Reid brought air into contact with a cold water spray in his modification of the heating and ventilating system of the British Parliament in 1836, and Charles Smyth experimented with air cycle cooling (1846 – 56), the problem was resolved by Willis Haviland Carrier’s U.S. Patent in 1906, in which he passed hot soggy air through a fine spray of water, condensing moisture on the droplets, leaving drier air behind. These inventions have had global implications.

Dr. Gorrie was honored by Florida, when his statue was placed in Statuary Hall in the U.S. Capitol. In 1899, a monument to Dr. Gorrie was erected by the Southern Ice Exchange in the small coastal town of Apalachicola, where he had served as mayor in 1837, and had developed his machine.

Reportedly born October 3, 1803 in Charleston, South Carolina, of Scots – Irish descent, he was raised in Columbia, S.C. He attended the College of Physicians and Surgeons of the Western District of New York, in Fairfield, New York, from 1825 to 1827. Although the school lasted only a few decades, it had a profound influence, second only to the Philadelphia Medical School, upon the scientific and medical community of the United States in the 19th century. Young Asa Gray, from Oneida County, New York, who by 1848 would be ranked as the leading botanist in the United States, and who in time would become a close friend of Dr. Alvin Wentworth Chapman of Apalachicola, the leading botanist in the South, served as an assistant in the school’s chemical department. In later years, Dr. Gray had distinct recollections of Gorrie as a “promising student.”

Dr. Gorrie initially practiced in Abbeville, South Carolina, in 1828, coming to the burgeoning cotton port of Apalachicola in 1833. He supplemented his income by becoming Assistant (1834), then Postmaster in Apalachicola. He became a Notary Public in 1835. The Apalachicola Land Company obtained clear title to the area by a U.S. Supreme Court decision in 1835, and in 1836 laid out the city’s grid-iron plat along the lines of Philadelphia, Pennsylvania. Gorrie, who served as Vice-Intendant in 1836, and Intendant (Mayor), in 1837, would be an effective advocate for the rest of his life for draining the swamps, clearing the weeds and maintaining clean food markets in the city. He first served as Secretary of the Masonic Lodge in 1835, was a partner in the Mansion House Hotel (1836), President of the Apalachicola Branch Bank of Pensacola (1836), a charter member of the Marine Insurance Bank of Apalachicola (1837), a physician for the Marine Hospital Service of the U.S. Treasury Department (1837 – 1844), and a charter incorporator and founding vestryman of Trinity Episcopal Church, Apalachicola (1837).

Dr. Gorrie married Caroline Frances Myrick Beman, of a Columbia, South Carolina family, the widowed proprietress of the Florida Hotel in Apalachicola, on May 8, 1838. Shortly thereafter, he resigned his various positions in Apalachicola, and the family left the city not to return until 1840. He was named Justice of the Peace in 1841, the same year that yellow fever struck the area.

Mal-aria, Italian, “bad air”, and yellow fever, prevailed in the hot, low-lying, tropical and sub-tropical areas where there was high humidity and rapid decomposition of vegetation. Noxious effluvium, or poisonous marsh gas was thought to be the cause. The “putrid” winds from marshy lowlands were regarded as deadly, especially at night. The specific causes were unknown, and although one had quinine for malaria, the gin and tonic of India, there was no cure nor preventive vaccine, for yellow fever. The legendary Flying Dutchman was founded on the story of a ship with yellow fever onboard.

Malaria would start with shaking and violent chills, followed by high fever, and a drenching sweat. Insidious, it could recur in the victim as well as kill. Yellow fever did not recur; one either died or survived. It came in mysterious, vicious waves, killing anywhere from 12 to 70 percent of its victims. It started with shivering, high fever, insatiable thirst, savage headaches, and severe back and leg pains. In a day or so, the restless patient would become jaundiced and turn yellow. In the terminal stages, the patient would spit up mouthfuls of dark blood, the terrifying “black vomit” (vomito negro), the body temperature would drop, the pulse fade, and the comatose patient, cold to the touch, would die in about 8 to 10 hours. So great was the terror, that the victims would be buried as quickly as possible. Areas would be quarantined, and yellow flags flown. Gauze would be hung over beds to filter air; handkerchiefs would be soaked in vinegar; garlic would be worn in shoes. Bed linens and compresses would be soaked in camphor; sulfur would be burned in outdoor smudge pots. Gunpowder would be burned, and cannons would be fired. And, later, when it was over, the cleaning and fumigating would occur.

It would not be until 1901 in Havana, Cuba, that Drs. Walter Reed, Carlos Finlay and William Crawford Gorgas, with others, would demonstrate conclusively that the Aedes Aegypti, or Stegomyia Fasciata mosquito was the carrier of the yellow fever virus. It would be about the same time that the English physician, Sir Ronald Ross in India, would correctly identify the Anopheles mosquito as the carrier of the malaria protozoa. As early as 1848, in Mobile, Alabama, however, Dr. Josiah Nott first suggested that mosquitos might be involved. The yellow fever epidemic of 1841, and the hurricane and tidal wave, known locally as the “Great Tide” of 1842, destroyed Apalachicola’s rival cotton port of St. Joseph some thirty miles to the west on the deep water sound of St. Joseph’s Bay. Using Florida’s first railway (1837) to transport cotton from the Apalachicola River, St. Joseph had hosted Florida’s Constitutional Convention in 1838.

Dr. Gorrie became convinced that cold was the healer. He noted that “Nature would terminate the fevers by changing the seasons.” Ice, cut in the winter in northern lakes, stored in underground ice houses, and shipped, packed in sawdust, around the Florida Keys by sailing vessel, in mid-summer could be purchased dockside on the Gulf Coast. In 1844, he began to write a series of articles in Apalachicola’s “Commercial Advertiser” newspaper, entitled, “On the prevention of Malarial Diseases”.

He used the Nom De Plume, “Jenner”, a tribute to Edward Jenner, (1749 – 1823), the discoverer of smallpox vaccine. According to these articles, he had constructed an imperfect refrigeration machine by May, 1844, carrying out a proposal he had advanced in 1842. All of Gorrie’s personal records were accidentally destroyed sometime around 1860.

“If the air were highly compressed, it would heat up by the energy of compression. If this compressed air were run through metal pipes cooled with water, and if this air cooled to the water temperature was expanded down to atmospheric pressure again, very low temperatures could be obtained, even low enough to freeze water in pans in a refrigerator box.” The compressor could be powered by horse, water, wind driven sails, or steampower.
Dr. Gorrie submitted his patent petition on February 27, 1848, three years after Florida became a state. In April of 1848, he was having one of his ice machines built in Cincinnati, Ohio, at the Cincinnati Iron Works, and in Octobcr, he demonstrated its operation. It was described in the Scientific American in September of 1849. On August 22, 1850, he received London Patent #13,124, and on May 6, 1851, U. S. Patent #8080. Although the mechanism produced ice in quantities, leakage and irregular performance sometimes impaired its operation. Gorrie went to New Orleans in search of venture capital to market the device, but either problems in product demand and operation, or the opposition of the ice lobby, discouraged backers. He never realized any return from his invention. Upon his death on June 29, 1855, he was survived by his wife Caroline (1805 – 1864), his son John Myrick (1838 – 1866), and his daughter, Sarah (1844 – 1908). Dr. Gorrie is buried in Gorrie Square in Apalachicola, his wife and son are buried-St. Luke’s-Episcopal Cemetery, Marianna, Florida, and his daughter, in Milton, Florida.

Historic Beer Birthday: Ludwig Narziß, a.k.a. Beer Pope

September 30, 2024 By Jay Brooks

Today is the birthday of Ludwig Narziß (or Narziss), who was also known as the “Beer Pope” (September 30, 1925-November 29, 2022). He was born in Munich, and was “one of the world’s most renowned brewing scientists and educators of the 20th century. For almost thirty years, “was a leading authority in his field at the Weihenstephan Center of Life and Food Science in Freising, outside Munich. Often called the Harvard of beer, the school, part of the Technical University of Munich, is closely tied to the Bavarian state-owned brewery with which it shares its name. Following an apprenticeship at the Tucher Brewery in Nuremberg, Narziss arrived at Weihenstephan as a student in 1948. There, he received degrees in brewing science and engineering and later completed a doctorate, writing a thesis on the influence of different yeast strains on beer quality. In 1958, he became the brewmaster at Munich’s Löwenbräu Brewery. But Weihenstephan didn’t let Narziss stray for long. Only 6 years later he joined the faculty at Weihenstephan, taking over the chair of Brewing Technology I. While instructing students in the science of beer making and conducting research on methods in Weihenstephan’s test brewery, Professor Narziss at times also served as the school’s dean, once from 1968 to 1970 and again in 1990. He also made time available to be a council member of the European Brewery Convention, as well as the organization’s president from 1979 to 1983. Narziss has authored and co-authored literally hundreds of papers as well as three seminal text books, Abriss der Bierbrauerei (An Outline of Beer Brewing) in 1972, Die Technologie der Malzbereitung (Technology of Malt Preparation) in 1976, and Die Technologie der Würzebereitung (Technology of Wort Preparation) in 1985. These books have gone through many revisions and editions and are still used as standard student textbooks today. Professor Narziss retired from Weihenstephan in 1992. He still lives in Freising and serves as Professor Emeritus at his venerable alma mater.”

This was his obituary, from Brauwelt, written by Lydia Junkersfeld:

Prof. Ludwig Narziß was born in Munich on September 30, 1925 and grew up in Nuremberg. As a young adult he personally experienced the Second World War. His father was an authorized signatory at the Lederer Brewery and had planned a commercial apprenticeship at the Tucher Brewery for his son, who had returned home from captivity. Of his own accord, Ludwig Narziß soon switched to an apprenticeship as a brewer, which appealed to him more – the right decision, as it turned out. Despite the difficult conditions in the brewery after the war, he successfully completed his apprenticeship after two years in October 1947 and in 1948 switched to the Faculty of Brewing in Weihenstephan to study, where a long career began.

From 1951 he initially worked for two years as a research assistant and management consultant in the field service of the research institute for brewing at the Bavarian State Trade Institute in Nuremberg, where he also met his wife Dorle. In 1953, Prof. Weinfurtner recruited him to the State Brewery Testing and Research Institute (today the Weihenstephan Research Centre for Brewing and Food Quality) in Weihenstephan and offered him the opportunity to study for a doctorate (topic: “The influence of the yeast variety on beer quality“) which he completed in 1956.

After completing his doctorate, Prof. Narziß first went back into business: he worked at the Löwenbrauerei in Munich as first brewmaster and authorized signatory until 1964, before he returned to Weihenstephan. Between 1964 and 1992 he held the Chair for Technology at Brewery 1 of the Technical University of Munich in Weihenstephan, where he trained and shaped generations of students. Many ground-breaking innovations in brewery technology and technology occurred during this time, which Prof. Narziß initiated and managed. There was hardly an area in the brewery that could not be further developed through research from his chair: raw material issues, developments in malting, in the brewhouse, in fermentation and storage or in filtration. It was a basic need of Prof. Narziß to solve practical problems through scientific research; whilst often about optimizing production, it was of course also about improving the quality of the product. One of the most important research topics from his time was the avoidance of spoilage from oxygen in beer.

Hundreds of graduate students and over 50 doctoral students have worked on various topics under his expert guidance. He was a “doctor father” to them, with whom some of the former doctoral students have remained in close contact to this day. What distinguished Prof. Ludwig Narziß was his closeness to people. Despite the great international recognition, he maintained his humble and lovable manner towards everyone. He challenged his employees – he was strict in this matter – but he also promoted them to the best of his ability.

Hundreds of Publications

Many books, including classics of brewery technology, and hundreds of publications and lectures have emerged from his scientific work. All of this has made him a world-renowned brewing scientist whose expertise was valued by authorities, associations and breweries around the world. Prof. Narziß has not only significantly shaped the faculty for brewing in Weihenstephan, but also the reputation of German brewing science in general at home and abroad. It was always important to him to ensure dialogue between the brewing industry and brewing science, between research and brewing practice. It is only logical that a research prize, the Ludwig Narziß Award for Brewing Science from BrewingScience, bears his name.

Charlie Bamfort, Graham Stewart and Ludwig.

International Recognition

Prof. Ludwig Narziß joined the Institute of Brewing in 1965, after being nominated by the late Norman Curtis. He was elected President of the EBC Council in 1981, with the 19th EBC Congress being held in 1983 on the South Bank in London where, in collaboration with his EBC/IOB colleagues, he played a major role in the organisation of this event and 1600 delegates attended it. During the course of this Congress he had the opportunity to co-author a poster presentation (they were still very much in their infancy at that time) entitled “Composition of Worts and Beers of High Temperature Wort Boiling Systems“.

Prof. Narziß also participated in one of the Institute‘s London Section Cambridge meetings, also with Norman Curtis who was the London Section‘s Chairman at the time. In addition, he was a participant in the Master Brewer Association of the Americas Ontario District One-Day Conferences that were held in Toronto each January and he also was a participant in a number of the Association‘s National Conferences.

Prof. Narziß was awarded the IBD’s Horace Brown Medal in 1990 and the accompanying lecture, 125 Years Research At Weihenstephan, was delivered at the Royal Society in London and published in the Journal of the Institute of Brewing in 1992.

Many Awards and Honours

Commitment to voluntary work at home and abroad was also a matter of course for him, for example his 1979 election as President of the European Brewery Convention (EBC) in Brussels. To this day he is the only honorary president of the EBC. Prof. Narziß was the bearer of the Cross of Merit of the Order of Merit of the Federal Republic of Germany as well as receiving many other honours and awards.

In 1992, Prof. Narziß handed over the management of the chair to Prof. Werner Back, but retained his research spirit, which kept him very active to the end. At the age of over 90, he was still traveling the world to attend brewing industry events and give lectures.

In spring 2021 he moved from Freising and Weihenstephan, the centre of his life for many years, to his family, who live near Reutlingen. Even if things got a little quieter around him, he still worked on his books every day, kept himself up to date with telephone calls.

The contribution given by Prof. Ludwig Narziß to the brewing industry internationally has been unique and we have lost a truly remarkable icon!

And this obituary is from Weinheistephaner:

Prof. Ludwig Narziß was a true “Weihenstephaner“: he passed his degree here after his brewery apprenticeship and also completed his doctorate in 1956. After his time as brewmaster at Löwenbräu, he was appointed to the Chair of Brewery Technology I and took over management of the teaching and research brewery. Until 1992, Prof. Narziß was the man at the helm, researching the further development of brewing – teaching the craft of brewing to thousands and thousands of students. Even today, many young people benefit from his works, which are held as standard works in brewing and beverage technology.

His professional expertise was more than appreciated by the Bavarian State Brewery Weihenstephan. Not only was great value placed here on his opinion on new beers – he was also a very welcome conversation partner at the table in the Bräustüberl. In every exchange, Prof. Narziß provided a different perspective, a further idea, a new approach: the fire for brewing was still burning in him until the very last moment. A characteristic that everyone appreciates to this day and that proved infectious to all his students.

Alongside all his professional expertise, it must never go unmentioned that with Prof. Narziß we lost a fine person. Never at a loss for a joke, always a smile on his lips – and above all he always had an open ear for every generation. Regardless of whether you were a young student or an experienced brewer: no question was left unanswered, Prof. Narziß always made time for you. He usually finished his meals in the Bräustüberl with a sip from his favorite beer, the Pils – and in the next days, people will be raising a toast to his memory and legacy everywhere in the brewing world.

Prof. Dr. Josef Schrädler, Director of the Bavarian State Brewery Weihenstephan explained on Wednesday: ”For me, Prof. Narziß was always an important dialog partner and advisor in strategic questions.“ But not only that: “I looked forward to every beer tasting with him – he was just an extremely likeable person.“

And Tobias Zollo, Technical Director and First Brewmaster can only confirm this: “Prof. Narziß treated everyone with respect, he was a true brewer.“ For Zollo and the brewmaster team, the many discussions they had with him on the last product developments about three years ago were especially valuable. Passing on knowledge – that was Prof. Narziß‘ mission in life. And he fulfilled this mission brilliantly.

Historic Beer Birthday: Johann Peter Griess

September 6, 2024 By Jay Brooks

Today is the birthday of Johann Peter Griess (September 6, 1829-August 30, 1888). He was born in Kirchhosbach (now part of Waldkappel), Germany. He was “an early pioneer of organic chemistry.” While known for his work on synthetic dyes, and he was the first to develop “the diazotization of aryl amines (the key reaction in the synthesis of the azo dyes), and a major figure in the formation of the modern dye industry.” He also “worked for more than a quarter of a century at the brewery of Samuel Allsopp and Sons in Burton upon Trent, which, owing to the presence of several notable figures and an increase in the scientific approach to brewing, became a significant centre of scientific enquiry in the 1870s and 1880s.”

This is his biography from his Wikipedia page:

After he finished at an agricultural private school, he joined the Hessian cavalry, but left the military shortly after. He started his studies at the University of Jena in 1850, but changed to the University of Marburg in 1851. During his student life he was several times sentenced to the Karzer (campus jail) and was also banned from the city for one year, during which time he listened to lectures of Justus Liebig at the Ludwig Maximilians University of Munich. After most of the family possession had been spent, he had to start working at the chemical factory of Oehler in Offenbach am Main in 1856. This was only possible after the recommendation of Hermann Kolbe, who was head of the chemistry department in Marburg. The devastating fire of 1857 ended the production of chemicals at the factory and a changed Peter Griess rejoined Hermann Kolbe at the University of Marburg. His new enthusiasm for chemistry yielded the discovery of diazonium salts in 1858. The discovery of a new class of chemicals convinced August Wilhelm von Hofmann to invite Griess to join him at his new position at the Royal College of Chemistry. During his time at the Royal College, he studied the reactions of nitrogen-rich organic molecules. It took him quite long to become accustomed to his new home in England, but the fact that he married in 1869 and founded a family made it clear that he did not intend to return to Germany, even though he was offered a position at the BASF. He left and started a position at the Samuel Allsopp & Sons brewery in 1862 where he worked until his retirement. His wife died after a long, severe illness in 1886; he survived her for two years and died on August 30, 1888. He is buried in Burton upon Trent.

In 1858 he described the Griess diazotization reaction which would form the basis for the Griess test for detection of Nitrite. Most of his work related to brewing remained confidential, but his additional work on organic chemistry was published by him in several articles.

And this short piece is from the journal “Brewery History,” from 2005. The article was called “The Brewing Connection in the Oxford Dictionary of National Biography: Part II,” and was written by Ray Anderson:

Another man whose activities extended far beyond his brewery work was Griess, (Johann) Peter (1829-1888), chemist to Samuel Allsopp & Sons for 26 years from 1862 until his death. Griess’s inclusion in the dictionary rests on his discovery and subsequent work on ‘a new and versatile chemical reaction which could provide a route to a wide range of new compounds’. These diazo compounds, so called because they contained two atoms of nitrogen per molecule, were to be widely utilised in the production of azo dyes, and the dictionary hails Griess’s synthesis of them as ‘perhaps the greatest single discovery in the history of the dyestuffs industry’. Historians of chemistry place Griess in the front rank of Victorian chemists.

And this is an Abstract from an article, entitled “Johann Peter Griess FRS (1829–88): Victorian brewer and synthetic dye chemist” is from “Notes and Records, The Royal Society Journal of the History of Science,” by Edwin and Andrew Yates:

The German organic chemist Johann Peter Griess (1829–88), who first developed the diazotization of aryl amines (the key reaction in the synthesis of the azo dyes), and a major figure in the formation of the modern dye industry, worked for more than a quarter of a century at the brewery of Samuel Allsopp and Sons in Burton upon Trent, which, owing to the presence of several notable figures and an increase in the scientific approach to brewing, became a significant centre of scientific enquiry in the 1870s and 1880s. Unlike the other Burton brewing chemists, Griess paralleled his work at the brewery with significant contributions to the chemistry of synthetic dyes, managing to keep the two activities separate—to the extent that some of his inventions in dye chemistry were filed as patents on behalf of the German dye company BASF, without the involvement of Allsopp’s. This seemingly unlikely situation can be explained partly by the very different attitudes to patent protection in Britain and in Germany combined with an apparent indifference to the significant business opportunity that the presence of a leading dye chemist presented to Allsopp’s. Although his work for the brewery remained largely proprietary, Griess’s discoveries in dye chemistry were exploited by the German dye industry, which quickly outpaced its British counterpart. One less well-known connection between brewing and synthetic dyes, and one that may further explain Allsopp’s attitude, is the use of synthetic dyes in identifying microorganisms—the perennial preoccupation of brewers seeking to maintain yield and quality. Developments of Griess’s original work continue to be applied to many areas of science and technology.

That’s just the abstract, of course, but you can read the whole article online.

Historic Beer Birthday: Hans Adolf Krebs

August 25, 2024 By Jay Brooks

Today is the birthday of Hans Adolf Krebs (August 25, 1900-November 22, 1981). He was a German-born British physician and biochemist. He was the pioneer scientist in study of cellular respiration, a biochemical pathway in cells for production of energy. He is best known for his discoveries of two important chemical reactions in the body, namely the urea cycle and the citric acid cycle. The latter, the key sequence of metabolic reactions that produces energy in cells, often eponymously known as the “Krebs cycle,” earned him a Nobel Prize in Physiology or Medicine in 1953. And it’s the Krebs cycle that is his relation to brewing, as it’s also known as the respiratory phase, the second aerobic state of the fermentation process immediately following the lag period.

Here’s a description of the Krebs cycle from Life Fermented:

The Krebs cycle, also known as the tricarboxylic acid (TCA) cycle or the citric acid cycle, is a circular and repeating set of reactions which requires oxygen. In beer making, this would occur in the first stage of fermentation when the yeast is pitched into a well aerated wort, and carries on until all oxygen is used up.
Pyruvate (are you tired of this word yet?) is first converted to acetyl-CoA (pronounced “Co-A”) in the following reaction:

pyruvate + 2 NAD+ + CoA-SH → acetyl-CoA + CO2 + NADH, with the help of the pyruvate dehydrogenase (PDH) complex. Note that this is the first time CO2 is produced, and yet more NADH is generated.

This acetyl-CoA then enters into a cycle of reactions which nets two molecules of CO2, one GTP (guanosine triphosphate, another unit of energy equivalent to ATP), three NADH, and one FADH2 (flavin adenine dinucleotide, which functions similarly to NADH). After the cycle completes, another acetyl-CoA molecule enters and the cycle repeats itself.

But wait, this just made more NADH, and we need to regenerate NAD+ so glycolysis can continue. Both the NADH and FADH2 now donate their electrons to a process called the electron transport chain/ oxidative phosphorylation. The result is a return of NAD to the NAD+ state, and a large amount of ATP cellular energy.

Because the Krebs cycle is so efficient at producing ATP energy units, this is the yeast’s preferred pathway. But, you’ll notice a rather conspicuous absence: ethanol. This is only formed in the absence of oxygen.

Here’s a biography of Krebs, from the Nobel Prize website:

Sir Hans Adolf Krebs was born at Hildesheim, Germany, on August 25th, 1900. He is the son of Georg Krebs, M.D., an ear, nose, and throat surgeon of that city, and his wife Alma, née Davidson.

Krebs was educated at the Gymnasium Andreanum at Hildesheim and between the years 1918 and 1923 he studied medicine at the Universities of Göttingen, Freiburg-im-Breisgau, and Berlin. After one year at the Third Medical Clinic of the University of Berlin he took, in 1925, his M.D. degree at the University of Hamburg and then spent one year studying chemistry at Berlin. In 1926 he was appointed Assistant to Professor Otto Warburg at the Kaiser Wilhelm Institute for Biology at Berlin-Dahlem, where he remained until 1930.

In I930, he returned to hospital work, first at the Municipal Hospital at Altona under Professor L. Lichtwitz and later at the Medical Clinic of the University of Freiburg-im-Breisgau under Professor S. J. Thannhauser.

In June 1933, the National Socialist Government terminated his appointment and he went, at the invitation of Sir Frederick Gowland Hopkins, to the School of Biochemistry, Cambridge, where he held a Rockefeller Studentship until 1934, when he was appointed Demonstrator of Biochemistry in the University of Cambridge.

In 1935, he was appointed Lecturer in Pharmacology at the University of Sheffield, and in 1938 Lecturer-in-Charge of the Department of Biochemistry then newly founded there.

In 1945 this appointment was raised to that of Professor, and of Director of a Medical Research Council’s research unit established in his Department. In 1954 he was appointed Whitley Professor of Biochemistry in the University of Oxford and the Medical Research Council’s Unit for Research in Cell Metabolism was transferred to Oxford.

Professor Krebs’ researches have been mainly concerned with various aspects of intermediary metabolism. Among the subjects he has studied are the synthesis of urea in the mammalian liver, the synthesis of uric acid and purine bases in birds, the intermediary stages of the oxidation of foodstuffs, the mechanism of the active transport of electrolytes and the relations between cell respiration and the generation of adenosine polyphosphates.

Among his many publications is the remarkable survey of energy transformations in living matter, published in 1957, in collaboration with H. L. Kornberg, which discusses the complex chemical processes which provide living organisms with high-energy phosphate by way of what is known as the Krebs or citric acid cycle.

Krebs was elected a Fellow of the Royal Society of London in 1947. In 1954 the Royal Medal of the Royal Society, and in 1958 the Gold Medal of the Netherlands Society for Physics, Medical Science and Surgery were conferred upon him. He was knighted in 1958. He holds honorary degrees of the Universities of Chicago, Freiburg-im-Breisgau, Paris, Glasgow, London, Sheffield, Leicester, Berlin (Humboldt University), and Jerusalem.

He married Margaret Cicely Fieldhouse, of Wickersley, Yorkshire, in 1938. They have two sons, Paul and John, and one daughter, Helen.

And in the Microbe Wiki, on a page entitled “Saccharomyces cerevisiae use and function in alcohol production,” under a section called “Fermentation of alchohol,” the Krebs cycle is placed in its portion in the fermentation process:

Saccharomyces cerevisiae is able to perform both aerobic and anaerobic respiration. The process begins with the yeast breaking down the different forms of sugar in the wort. The types of sugars typically found in wort are the monosaccharides glucose and fructose. These sugars contain a single hexose, which is composed of 6 carbon atoms in the molecular formula C6H12O6. Disaccharides are formed when two monosaccharides join together. Typical disaccharides in the wort are galactose, sucrose, and maltose. The third type of fermentable sugar in the wort is a trisaccharide. This trisaccharide is formed when three monosccharides join together. Maltotriose is the trisaccharide commonly found in the wort and is composed of three glucose molecules. The wort does contain other sugars such as dextrins but it is not fermentable by yeast10. These dextrins contain four monosaccarides joined together. In order for the yeast to use the disaccharides and trisaccharides they first must be broken down to monosaccharides. The yeast does this by using different enzymes both inside and outside the cell. The enzyme invertase is used to break down sucrose into glucose and fructose. The invertase catalyzes the hydrolysis of the sucrose by breaking the O-C (fructose bond). The other enzyme used is maltase, which breaks down maltose and maltotriose into glucose inside the cell. The enzyme does this by catalyzing the hydrolysis of the sugars by breaking the glycosidic bond holding the glucose molecules together.

Once the sugars are broken down into monosaccharides the yeast can use them. The primary step is called glycolysis. In this process the glucose is converted to pyruvate using different enzymes in a series of chemical modifications. The electrons from glucose end up being transferred to energy carrying molecules like NAD+ to form NADH. ATP is also formed when phosphates are transferred from high-energy intermediates of glycolysis to ADP. In the presence of oxygen aerobic respiration can occur. This occurs in the mitochondria of the yeast. The energy of the pyruvate is extracted when it goes through metabolic processes like the Krebs cycle. The products of this type of metabolism are ATP, H2O, and CO2. However if there is no oxygen present and an abundance of sugars, as in the wort, the yeast undergo alcoholic fermentation. This type of metabolism yields much smaller amounts of energy when compared to aerobic respiration. However, because of the large supply of sugars from the different grains the wort is a very good environment for fermentative growth. The alcoholic fermentation begins with the two pyruvate acquired from glycolysis. These two pyruvate are decarboxylated by pyruvate decarboxylase to form two acetaldehydes and CO2. The CO2 is the gas that is observed during fermentation as bubbles that float to the top of the wort creating the kräusen or beer head, the foam that is very characteristic of a freshly poured beer. Pyruvate decarboxylase is a homotetramer meaning it contains four identical subunits. This also means that is has four active sites. The active sites are where the pyruvate reacts with the cofactors thiamine pyrophosphate (TPP) and magnesium to remove the carbon dioxide9. The final step to form alcohol is the addition of a hydrogen ion to the aldehyde to form ethanol. This hydrogen ion is from the NADH made during glycolysis and converts back to NAD+. The ethanol is originally believed to serve as an antibiotic against other microbes. This form of defense ensures that bacteria do not grow in the wort, thus ruining the beer with off flavors. However recently with the boom of craft beer different bacteria have been purposefully added to create what is known as sour beer. The sour taste comes from the waste products of the bacteria.

krebs_cycle_from_wikimedia-tweaked

To learn more about the Krebs cycle check out this video from the University of Oklahoma’s Chemistry of Beer – Unit 7 – Chemical Concepts: Krebs Cycle:

Historic Beer Birthday: Johan Kjeldahl

August 16, 2024 By Jay Brooks

carlsberg-crown
Today is the birthday of Johan Gustav Christoffer Thorsager Kjeldahl (August 16, 1849-July 18, 1900) He was a Danish chemist who developed a method for determining the amount of nitrogen in certain organic compounds using a laboratory technique which was named the Kjeldahl method after him.

Kjeldahl worked in Copenhagen at the Carlsberg Laboratory, associated with Carlsberg Brewery, where he was head of the Chemistry department from 1876 to 1900.

He was given the job to determine the amount of protein in the grain used in the malt industry. Less protein meant more beer. Kjeldahl found the answer was in developing a technique to determine nitrogen with accuracy but existing methods in analytical chemistry related to proteins and biochemistry at the time were far from accurate.

Haslund_Johan_Kjeld A painting by Otto Haslund of Johan Kjeldahl.

His discovery became known as the Kjeldahl Method

Kjeldahl's_distillation

The method consists of heating a substance with sulphuric acid, which decomposes the organic substance by oxidation to liberate the reduced nitrogen as ammonium sulphate. In this step potassium sulphate is added to increase the boiling point of the medium (from 337 °C to 373 °C) . Chemical decomposition of the sample is complete when the initially very dark-coloured medium has become clear and colourless.

The solution is then distilled with a small quantity of sodium hydroxide, which converts the ammonium salt to ammonia. The amount of ammonia present, and thus the amount of nitrogen present in the sample, is determined by back titration. The end of the condenser is dipped into a solution of boric acid. The ammonia reacts with the acid and the remainder of the acid is then titrated with a sodium carbonate solution by way of a methyl orange pH indicator.

In practice, this analysis is largely automated; specific catalysts accelerate the decomposition. Originally, the catalyst of choice was mercuric oxide. However, while it was very effective, health concerns resulted in it being replaced by cupric sulfate. Cupric sulfate was not as efficient as mercuric oxide, and yielded lower protein results. It was soon supplemented with titanium dioxide, which is currently the approved catalyst in all of the methods of analysis for protein in the Official Methods and Recommended Practices of AOAC International.

And Velp Scientifica also has an explanation of his method, which is still in use today.

Kjeldahl (center) in his laboratory.

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